Source:http://linkedlifedata.com/resource/pubmed/id/20061417
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2010-3-5
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| pubmed:abstractText |
Context: Micro-RNAs (miRNAs) have been recently involved in the modulation of several biological activities including cancer. Many human tumors show deregulated expression of miRNAs targeting oncogenes and/or tumor suppressors, thus identifying miRNAs as new molecular targets for cancer therapy. Objectives: Nuclear factor (NF)-kappaB is strongly activated in human anaplastic thyroid carcinomas (ATCs). Because the regulation of miRNA expression is under control of RNA polymerase II-dependent transcription factors, we stably inactivated NF-kappaB in the ATC-derived FRO cell line and analyzed its miRNA profile in comparison with the parental counterpart by using a miRNA chip microarray. Results: The analysis revealed that a number of miRNAs were differentially expressed in the two cell lines. Among others, the miR-146a showed a strong down-regulation that was confirmed by quantitative real time RT-PCR. The expression of miR-146a was almost undetectable in mouse embryonic fibroblasts isolated from the RelA knockout mice and was restored after reexpression of RelA, thus indicating that miR-146a transcription was controlled by NF-kappaB. The inhibition of miR-146a expression in FRO cells decreased their oncogenic potential and increased the susceptibility to chemotherapeutic drug-induced apoptosis. No difference was found in the growth rate between untransfected and miR-146a-null FRO cells. Importantly, the miR-146a resulted in overexpression of human ATC specimens compared with the normal thyroid tissue. Conclusions: Our results show that NF-kappaB contributes to anaplastic thyroid cancer up-regulating the expression of miR-146a.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
1945-7197
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| pubmed:author |
pubmed-author:CrescenziElviraE,
pubmed-author:FormisanoSilvestroS,
pubmed-author:GriecoMicheleM,
pubmed-author:IannettiAlessioA,
pubmed-author:LeonardiAntonioA,
pubmed-author:LiguoroDomenicoD,
pubmed-author:MelloneStefanoS,
pubmed-author:MoscatoFortunatoF,
pubmed-author:PacificoFrancescoF,
pubmed-author:PorrinoNunzioN
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| pubmed:issnType |
Electronic
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| pubmed:volume |
95
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1421-30
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| pubmed:meshHeading |
pubmed-meshheading:20061417-Analysis of Variance,
pubmed-meshheading:20061417-Animals,
pubmed-meshheading:20061417-Apoptosis,
pubmed-meshheading:20061417-Blotting, Western,
pubmed-meshheading:20061417-Carcinoma,
pubmed-meshheading:20061417-Cell Line, Tumor,
pubmed-meshheading:20061417-Cell Proliferation,
pubmed-meshheading:20061417-Cells, Cultured,
pubmed-meshheading:20061417-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:20061417-Humans,
pubmed-meshheading:20061417-Immunohistochemistry,
pubmed-meshheading:20061417-Mice,
pubmed-meshheading:20061417-MicroRNAs,
pubmed-meshheading:20061417-Microarray Analysis,
pubmed-meshheading:20061417-NF-kappa B,
pubmed-meshheading:20061417-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:20061417-Thyroid Neoplasms,
pubmed-meshheading:20061417-Up-Regulation
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| pubmed:year |
2010
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| pubmed:articleTitle |
Nuclear factor-{kappa}B contributes to anaplastic thyroid carcinomas through up-regulation of miR-146a.
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| pubmed:affiliation |
Dipartimento di Biologia e Patologia Cellulare e Molecolare, "Federico II" University of Naples, Via S. Pansini 5, 80131 Naples, Italy.
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| pubmed:publicationType |
Journal Article
|