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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2010-1-11
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pubmed:abstractText |
Host-defense mechanisms against transposable elements are critical to protect the genome information. Here we show that tudor-domain containing 9 (Tdrd9) is essential for silencing Line-1 retrotransposon in the mouse male germline. Tdrd9 encodes an ATPase/DExH-type helicase, and its mutation causes male sterility showing meiotic failure. In Tdrd9 mutants, Line-1 was highly activated and piwi-interacting small RNAs (piRNAs) corresponding to Line-1 were increased, suggesting that feedforward amplification operates in the mutant. In fetal testes, Tdrd9 mutation causes Line-1 desilencing and an aberrant piRNA profile in prospermatogonia, followed by cognate DNA demethylation. TDRD9 complexes with MIWI2 with distinct compartmentalization in processing bodies, and this TDRD9-MIWI2 localization is regulated by MILI and TDRD1 residing at intermitochondrial cement. Our results identify TDRD9 as a functional partner of MIWI2 and indicate that the tudor-piwi association is a conserved feature, while two separate axes, TDRD9-MIWI2 and TDRD1-MILI, cooperate nonredundantly in the piwi-small RNA pathway in the mouse male germline.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1878-1551
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pubmed:author |
pubmed-author:ChujoTakeshiT,
pubmed-author:ChumaShinichiroS,
pubmed-author:HataKenichiroK,
pubmed-author:HosokawaMihokoM,
pubmed-author:KatoYuzuruY,
pubmed-author:KondohGenG,
pubmed-author:Kuramochi-MiyagawaSatomiS,
pubmed-author:MartinSandra LSL,
pubmed-author:NakanoToruT,
pubmed-author:NakatsujiNorioN,
pubmed-author:NoceToshiakiT,
pubmed-author:OkawaKatsuyaK,
pubmed-author:PillaiRamesh SRS,
pubmed-author:ReuterMichaelM,
pubmed-author:SasakiHiroyukiH,
pubmed-author:ShojiMasanobuM,
pubmed-author:StarkAlexanderA,
pubmed-author:SuzukiTsutomuT,
pubmed-author:TanakaTakashiT
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pubmed:copyrightInfo |
2009 Elsevier Inc. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
775-87
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:20059948-Amino Acid Sequence,
pubmed-meshheading:20059948-Animals,
pubmed-meshheading:20059948-Argonaute Proteins,
pubmed-meshheading:20059948-Chromosome Pairing,
pubmed-meshheading:20059948-DNA Helicases,
pubmed-meshheading:20059948-Female,
pubmed-meshheading:20059948-Infertility, Male,
pubmed-meshheading:20059948-Long Interspersed Nucleotide Elements,
pubmed-meshheading:20059948-Male,
pubmed-meshheading:20059948-Meiosis,
pubmed-meshheading:20059948-Mice,
pubmed-meshheading:20059948-Protein Structure, Tertiary,
pubmed-meshheading:20059948-Proteins,
pubmed-meshheading:20059948-RNA, Small Interfering,
pubmed-meshheading:20059948-RNA-Binding Proteins,
pubmed-meshheading:20059948-Spermatocytes
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pubmed:year |
2009
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pubmed:articleTitle |
The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline.
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pubmed:affiliation |
Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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