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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1991-4-22
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pubmed:abstractText |
We have analyzed the biosynthesis of human fibroblast growth factor-5 (FGF-5) at the translational and posttranslational levels. FGF-5 RNA synthesized in vitro can be translated in rabbit reticulocyte lysates to yield a 29,500-Da protein, which is consistent with the molecular weight predicted from the coding sequence. The efficiency of FGF-5 translation is dramatically enhanced if an upstream open reading frame (ORF-1) in the RNA is deleted or if both AUG codons in ORF-1 are destroyed by point mutations, while partial enhancement is achieved by individual mutation of either ORF-1 AUG codon. These data suggest that FGF-5 synthesis requires the scanning of ribosomes past the two ORF-1 AUG codons. The introduction of these ORF-1 mutations into a eukaryotic FGF-5 expression vector increases its capacity to transform mouse NIH 3T3 cells up to 50-fold upon transfection. FGF-5 is secreted from transfected 3T3 cells and from human tumor cells as glycoproteins containing heterogeneous amounts of sialic acid. Glycosidase treatments suggest that the growth factor bears both N-linked and O-linked sugars.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-1700424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-1963077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-222468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-2318343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-2406607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-2475908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-2538817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-2549857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-2557543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-2645293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-2726761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-3003063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-3043199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-3088566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-3211147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-3323813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-3330780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-3347254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-3392047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-3409320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-3516411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-3683388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-3943125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-6091052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-6159641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-6167985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-823012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2005884-90521
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1840-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2005884-Amino Acid Sequence,
pubmed-meshheading:2005884-Animals,
pubmed-meshheading:2005884-Base Sequence,
pubmed-meshheading:2005884-Cell Line,
pubmed-meshheading:2005884-Cell Transformation, Neoplastic,
pubmed-meshheading:2005884-Fibroblast Growth Factor 5,
pubmed-meshheading:2005884-Fibroblast Growth Factors,
pubmed-meshheading:2005884-Glycosylation,
pubmed-meshheading:2005884-Humans,
pubmed-meshheading:2005884-Immunoblotting,
pubmed-meshheading:2005884-Mice,
pubmed-meshheading:2005884-Molecular Sequence Data,
pubmed-meshheading:2005884-Molecular Weight,
pubmed-meshheading:2005884-Mutation,
pubmed-meshheading:2005884-Open Reading Frames,
pubmed-meshheading:2005884-Protein Biosynthesis,
pubmed-meshheading:2005884-Rabbits,
pubmed-meshheading:2005884-Transfection,
pubmed-meshheading:2005884-Tumor Cells, Cultured
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pubmed:year |
1991
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pubmed:articleTitle |
Biosynthesis of human fibroblast growth factor-5.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biophysics, Columbia University College of Physicians and Surgeons, New York, New York 10032.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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