Linked Life Data

20058085

Source:http://linkedlifedata.com/resource/pubmed/id/20058085

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-3-19
pubmed:abstractText
Selective protein channels in cell and nuclear membranes act as gateways to control the passage of molecules across. The selectivity of these channels stems from attractive potentials of the binding sites in the transmembrane proteins. These channels can filter out small volume of solutions with high precision. Motivated from this phenomenon, we report biomimetic facilitated transport modality to selectively separate a target molecule from a mixture of molecules. The attractive potential is generated by specific antibodies immobilized inside 15 nm diameter polycarbonate nanochannels. Two proteins with similar physicochemical properties (Bovine Serum Albumin 66 kDa, and Human Hemoglobin 65 kDa) are chosen as model molecules. The protein molecules are mixed in ratios of 1:1, 1:20 and 1:40 (Hb:BSA), and separation of molecules is demonstrated. The selectivity of membrane can be switched from Hb to BSA by changing the immobilized antibody inside the membrane channels. This approach can be used to selectively enrich any target molecule from a complex sample to enhance signal-to-noise ratio for early disease diagnosis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1572-8781
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
317-24
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Active and biomimetic nanofilters for selective protein separation.
pubmed:affiliation
Department of Bioengineering, University of Texas at Arlington,Arlington, TX 76019, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.