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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1978-1-27
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pubmed:abstractText |
A somatic cell genetic approach was used to study the role of cyclic nucleotides in adrenal steroidogenesis. 8-Bromoadenosine 3',5'-monophosphate (8BrcAMP) stimulated steroidogenesis (K'd=0.1 mM) in cultured mouse adrenocortical tumor cells (Clone Y1). In addition, 8BrcAMP inhibited Y1 cell growth and caused Y1 cell monolayers to assume a rounded morphology. As a consequence, 8BrcAMP (at concentrations greater than or equal to 0.4 mM) reduced the relative plating efficiency of Y1 cells to less than 10(-5). Y1 cells were mutagenized with ethyl methanesulfonate (300 microgram/ml) and grown in the presence of 0.4 mM 8BrcAMP. A surviving colony (8BrcAMPr-1) was shown to be resistant to growth inhibition (relative plating efficiency at 1.0 mM 8BrcAMP=50 percent)) and to morphological changes induced by 8BrcAMP. 8BrcAMPr-1 cells had diminished steroidogenic responses to cyclic nucleotides and to ACTH (less than or equal to 33 percent of maximum). In 8BrcAMP(R)-1 cells, adenylate cyclase activity remained responsive to ACTH, and cyclic AMP phosphodiesterase activity was not increased. These data suggest that 8BrcAMPr-1 cells are defective at a point common to cyclic AMP action on growth, morphology and steroidogenesis. The associated decrease in responsiveness of the steroidogenic pathway to ACTH suggests that ACTH-regulated steroidogenesis is via a cyclic nucleotide-mediated mechanism.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3',5'-Cyclic-AMP Phosphodiesterases,
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Adrenocorticotropic Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Bromine,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Steroids,
http://linkedlifedata.com/resource/pubmed/chemical/Xanthines
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0303-7207
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
135-45
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:200506-3',5'-Cyclic-AMP Phosphodiesterases,
pubmed-meshheading:200506-Adenylate Cyclase,
pubmed-meshheading:200506-Adrenal Cortex Neoplasms,
pubmed-meshheading:200506-Adrenal Gland Neoplasms,
pubmed-meshheading:200506-Adrenocorticotropic Hormone,
pubmed-meshheading:200506-Animals,
pubmed-meshheading:200506-Bromine,
pubmed-meshheading:200506-Cell Survival,
pubmed-meshheading:200506-Cells, Cultured,
pubmed-meshheading:200506-Cyclic AMP,
pubmed-meshheading:200506-DNA, Neoplasm,
pubmed-meshheading:200506-Drug Resistance,
pubmed-meshheading:200506-Mice,
pubmed-meshheading:200506-Mutation,
pubmed-meshheading:200506-Steroids,
pubmed-meshheading:200506-Xanthines
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pubmed:year |
1977
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pubmed:articleTitle |
Isolation of mutant adrenocortical tumor cells resistant to cyclic nucleotides.
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pubmed:publicationType |
Journal Article
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