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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2010-2-9
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pubmed:abstractText |
CLIC1 belongs to a family of highly conserved and widely expressed intracellular chloride ion channel proteins existing in both soluble and membrane integrated forms. To study the physiological and biological role of CLIC1 in vivo, we undertook conditional gene targeting to engineer Clic1 gene knock-out mice. This represents creation of the first gene knock-out of a vertebrate CLIC protein family member. We first generated a Clic1 Knock-in (Clic1(FN)) allele, followed by Clic1 knock-out (Clic1(-/-)) mice by crossing Clic1(FN) allele with TNAP-cre mice, resulting in germline gene deletion through Cre-mediated recombination. Mice heterozygous or homozygous for these alleles are viable and fertile and appear normal. However, Clic1(-) (/-) mice show a mild platelet dysfunction characterized by prolonged bleeding times and decreased platelet activation in response to adenosine diphosphate stimulation linked to P2Y(12) receptor signaling.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1526-968X
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pubmed:author |
pubmed-author:BreitSamuel NSN,
pubmed-author:BrownLouise JLJ,
pubmed-author:ConnorDavidD,
pubmed-author:CurmiPaul M GPM,
pubmed-author:JacksonShaun PSP,
pubmed-author:JiangLeleL,
pubmed-author:JosephJoanne EJE,
pubmed-author:KuffnerTamaraT,
pubmed-author:LowJoyceJ,
pubmed-author:Mahaut-SmithMartynM,
pubmed-author:ManginPierreP,
pubmed-author:MatthaeiKlaus IKI,
pubmed-author:QiuMin RuMR,
pubmed-author:SchoenwaelderSimone MSM,
pubmed-author:ValenzuelaStella MSM
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pubmed:copyrightInfo |
(c) 2010 Wiley-Liss, Inc.
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pubmed:issnType |
Electronic
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pubmed:volume |
48
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
127-36
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pubmed:dateRevised |
2010-5-26
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pubmed:meshHeading |
pubmed-meshheading:20049953-Alleles,
pubmed-meshheading:20049953-Animals,
pubmed-meshheading:20049953-Blood Platelets,
pubmed-meshheading:20049953-Chloride Channels,
pubmed-meshheading:20049953-Crosses, Genetic,
pubmed-meshheading:20049953-Gene Deletion,
pubmed-meshheading:20049953-Gene Targeting,
pubmed-meshheading:20049953-Genetic Engineering,
pubmed-meshheading:20049953-Hemorrhage,
pubmed-meshheading:20049953-Heterozygote,
pubmed-meshheading:20049953-Homozygote,
pubmed-meshheading:20049953-Immunohistochemistry,
pubmed-meshheading:20049953-Integrases,
pubmed-meshheading:20049953-Mice,
pubmed-meshheading:20049953-Mice, Knockout,
pubmed-meshheading:20049953-Models, Genetic,
pubmed-meshheading:20049953-Recombination, Genetic
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pubmed:year |
2010
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pubmed:articleTitle |
Generation and characterization of mice with null mutation of the chloride intracellular channel 1 gene.
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pubmed:affiliation |
St. Vincent's Centre for Applied Medical Research, St. Vincent's Hospital and University of New South Wales, Sydney, New South Wales, Australia.
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pubmed:publicationType |
Journal Article
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