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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-1-4
pubmed:abstractText
Nitric oxide (NO) and the partial pressure of oxygen (pO(2)) in the liver were simultaneously quantified in rats with partial hepatic ischemia/reperfusion injury (PHIRI). Real-time NO/pO(2) monitoring and immunohistochemical analysis for superoxide dismutase and inducible nitric oxide synthase (iNOS) and endothelial NOS (eNOS) were performed to evaluate the protective effects of a dihydropyridine-type calcium-channel blocker--CV159--on PHIRI. Serum high-mobility-group box-1 (HMGB-1) was measured to assess cellular necrosis. Moreover, we used in vitro/ex vivo electron paramagnetic resonance spin trapping to assess the hydroxyl radical (*OH)-scavenging activity (OHSA) of CV159 and the liver tissue. The NO levels were significantly higher in CV159-treated rats than in control rats throughout the ischemic phase. Immediately after reperfusion, the levels temporarily increased in waves and then gradually decreased in the treated rats but remained constant in the control rats. pO(2) was continually higher in the treated rats. In these rats, hepatic eNOS expression increased, whereas iNOS expression decreased. The treated rats exhibited significantly higher cytosolic and mitochondrial concentrations NOx (NO(2)+NO(3)). The serum HMGB-1 levels significantly decreased in the treated rats. Moreover, CV159 directly scavenged *OH and both mitochondrial and cytosolic OHSA were preserved in the treated rats. Thus, CV159-mediated inhibition of intracellular Ca(2+) overloading may effectively minimize organ damage and also have *OH-scavenging activity and the cytoprotective effects of eNOS-derived NO.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1347-5215
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
77-83
pubmed:meshHeading
pubmed-meshheading:20045940-Animals, pubmed-meshheading:20045940-Calcium Channel Blockers, pubmed-meshheading:20045940-Cytosol, pubmed-meshheading:20045940-Dihydropyridines, pubmed-meshheading:20045940-Electrodes, pubmed-meshheading:20045940-Electron Spin Resonance Spectroscopy, pubmed-meshheading:20045940-Endothelium, pubmed-meshheading:20045940-Free Radical Scavengers, pubmed-meshheading:20045940-HMGB1 Protein, pubmed-meshheading:20045940-Hydroxyl Radical, pubmed-meshheading:20045940-Liver, pubmed-meshheading:20045940-Male, pubmed-meshheading:20045940-Mitochondria, pubmed-meshheading:20045940-Necrosis, pubmed-meshheading:20045940-Nitric Oxide, pubmed-meshheading:20045940-Nitric Oxide Synthase, pubmed-meshheading:20045940-Nitrogen Oxides, pubmed-meshheading:20045940-Oxygen, pubmed-meshheading:20045940-Rats, pubmed-meshheading:20045940-Rats, Sprague-Dawley, pubmed-meshheading:20045940-Reperfusion Injury
pubmed:year
2010
pubmed:articleTitle
Effects of a calcium-channel blocker (CV159) on hepatic ischemia/reperfusion injury in rats: evaluation with selective NO/pO2 electrodes and an electron paramagnetic resonance spin-trapping method.
pubmed:affiliation
Department of Gastroenterological and General Surgery, St. Marianna University Hospital, Kawasaki, Japan.
pubmed:publicationType
Journal Article