Linked Life Data

20043016

Source:http://linkedlifedata.com/resource/pubmed/id/20043016

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2010-4-15
pubmed:abstractText
Cholesterol is organized in distinctive liquid-ordered micro-domains within biological membranes called lipid rafts. These micro-domains direct multiple physiological functions in mammalian cells by modulating signaling processes. Recent findings suggest a role for lipid rafts in cellular processes in human keratinocytes such as early differentiation and apoptosis. However, research of lipid rafts is hindered by technological limitations in visualizing dynamic cholesterol organization in plasma membranes. This study addresses a real-time, non-invasive method for the long-term observation of cholesterol reorganization in plasma membranes. In addition, this study also addresses the dynamic process of cholesterol depletion and repletion in primary human keratinocytes. Cholesterol reorganization was measured by observed changes in cellular impedance. Disruption of lipid rafts with low concentrations of methyl-beta-cyclodextrin (MbetaCD) resulted in an increase in the proliferative capacity of keratinocytes, which was assessed using real-time proliferation curves and adenosine triphosphate (ATP)-based proliferation assays. Quantitative PCR showed a concomitant decrease in messenger RNA (mRNA) expression of the early differentiation markers keratins 1 and 10. Conversely, specific cholesterol reintegration led to a 4.5-fold increase in keratin 2 mRNA expression, a marker for late keratinocyte differentiation, whereas depletion resulted in a significant downregulation. These findings imply a strictly controlled mechanism for the regulation of membrane cholesterol composition in both early and terminal keratinocyte differentiation. The impedance-based method that this study addresses further enhances our understanding of how physiological processes in keratinocytes are controlled by membrane cholesterol.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1523-1747
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
130
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1268-78
pubmed:meshHeading
pubmed-meshheading:20043016-Calcium, pubmed-meshheading:20043016-Cell Culture Techniques, pubmed-meshheading:20043016-Cell Differentiation, pubmed-meshheading:20043016-Cell Division, pubmed-meshheading:20043016-Cell Membrane, pubmed-meshheading:20043016-Cells, Cultured, pubmed-meshheading:20043016-Cholesterol, pubmed-meshheading:20043016-Electric Impedance, pubmed-meshheading:20043016-Epidermis, pubmed-meshheading:20043016-Filipin, pubmed-meshheading:20043016-Fluorescence Recovery After Photobleaching, pubmed-meshheading:20043016-Humans, pubmed-meshheading:20043016-Keratin-1, pubmed-meshheading:20043016-Keratin-10, pubmed-meshheading:20043016-Keratin-2, pubmed-meshheading:20043016-Keratinocytes, pubmed-meshheading:20043016-Membrane Microdomains, pubmed-meshheading:20043016-RNA, Messenger, pubmed-meshheading:20043016-Signal Transduction, pubmed-meshheading:20043016-beta-Cyclodextrins
pubmed:year
2010
pubmed:articleTitle
Real-time monitoring of membrane cholesterol reveals new insights into epidermal differentiation.
pubmed:affiliation
Research and Development, Beiersdorf AG, Hamburg, Germany.
pubmed:publicationType
Journal Article