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pubmed:abstractText |
The serine/threonine kinase PKB/Akt plays essential role in various cellular processes including cell growth and proliferation, metabolism and cell survival. The importance of the Akt pathway is highlighted by the mutation of various components of the pathway such as the PTEN and PI3-kinase (P110alpha) in human cancers. In this paper, we employed an RNA interference library targeting all human kinases to screen for kinases involved in the regulation of Akt activation, in particular serine 473 phosphorylation. Here, we transfected the MDA-MB 468 breast cell line with the human kinome siRNA library and measured Akt activation using an antibody specific for phosphoserine 473 of Akt.
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pubmed:affiliation |
Singapore OncoGenome Project, Institute of Medical Biology, A*STAR, 8A Biomedical Grove, 06-06 Immunos, Singapore. boontin.chua@imb.a-star.edu.sg
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