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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1991-4-23
|
| pubmed:abstractText |
We report an efficient procedure to synthesize either single- or double-stranded probes labeled with biotin-11-dUTP, biotin-21-dUTP or digoxigenin-11-dUTP. To produce the single-stranded probe, only a single primer is utilized in a Taq polymerase amplification of 55 cycles. A cytomegalovirus probe is presented. This procedure allows easy production of nonradioactively labeled pure single-stranded probes of any desired length and specificity.
|
| pubmed:grant | |
| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0736-6205
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
10
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
35-6, 38-9
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:2003918-Base Sequence,
pubmed-meshheading:2003918-DNA, Single-Stranded,
pubmed-meshheading:2003918-DNA Probes,
pubmed-meshheading:2003918-DNA-Directed DNA Polymerase,
pubmed-meshheading:2003918-Molecular Sequence Data,
pubmed-meshheading:2003918-Polymerase Chain Reaction,
pubmed-meshheading:2003918-Taq Polymerase
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Producing single-stranded DNA probes with the Taq DNA polymerase: a high yield protocol.
|
| pubmed:affiliation |
Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
|
| pubmed:publicationType |
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Technical Report
|