Linked Life Data

20034859

Source:http://linkedlifedata.com/resource/pubmed/id/20034859

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
2010-2-4
pubmed:abstractText
A new simple, rapid, sensitive and accurate quantitative detection method using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) for the measurement of formononetin (FMN) and daidzein (DZN) levels in rat plasma is described. Analytes were separated on a Supelco Discovery C18 (4.6x50 mm, 5.0 microm) column with acetonitrile: methanol (50:50, v/v) and 0.1% acetic acid in the ratio of 90:10 (v/v) as a mobile phase. The method was proved to be accurate and precise at linearity range of 5-100 ng/mL with a correlation coefficient (r) of > or = 0.996. The intra- and inter-day assay precision ranged from 1.66-6.82% and 1.87-6.75%, respectively; and intra- and inter-day assay accuracy was between 89.98-107.56% and 90.54-105.63%, respectively for both the analytes. The lowest quantitation limit for FMN and DZN was 5.0 ng/mL in 0.1 mL of rat plasma. Practical utility of this new LC-MS/MS method was demonstrated in a pharmacokinetic study in rats following intravenous administration of FMN.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1873-376X
pubmed:author
pubmed:copyrightInfo
Crown Copyright 2009. Published by Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
878
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
391-7
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Quantitative determination of formononetin and its metabolite in rat plasma after intravenous bolus administration by HPLC coupled with tandem mass spectrometry.
pubmed:affiliation
Pharmacokinetics and Metabolism Division, Central Drug Research Institute, CSIR, Lucknow 226001 Uttar Pradesh, India.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't