Linked Life Data

20026129

Source:http://linkedlifedata.com/resource/pubmed/id/20026129

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-3-15
pubmed:abstractText
Interleukin-6 (IL6) signals are mediated by classic and trans-signaling. In classic signaling, IL6 first binds to the membrane bound Interleukin-6 Receptor (IL6R) whereas in trans-signaling, IL6 acts via a soluble form of the IL6R. Trans-signaling via the soluble IL6R (sIL6R) was linked to chronic inflammation and cancer. The release of the IL6R is mediated by the disintegrin and metalloproteinases ADAM10 and ADAM17. To analyze the fate of the C-terminal cleavage fragment after ectodomain shedding we fused the IL6R C-terminally to two Z-domains of Protein-A (2Z-tag) or to GFP. A specific C-terminal fragment of the IL6R protein could be detected after ADAM17-induced shedding. Using gamma-secretase inhibitors and gene-deficient cells, we demonstrate that after ADAM17 mediated cleavage, the IL6R C-terminal fragment was cleaved by the gamma-secretase at the plasma membrane. We were, however, not able to detect an IL6R intracellular domain. After gamma-secretase cleavage IL6R cell surface expression was lost and gamma-secretase cleavage product(s) of the IL6R were endocytosed. No GFP-fluorescence of a gamma-secretase-cleaved IL6R-GFP fusion protein was observed in the nucleus. We therefore hypothesize that a potential IL6R intracellular domain fragment is not involved in nuclear signaling but rapidly degraded.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:volume
1803
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
234-45
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
ADAM17-mediated shedding of the IL6R induces cleavage of the membrane stub by gamma-secretase.
pubmed:affiliation
Institute of Biochemistry, Christian-Albrechts-University of Kiel, Olshausenstr. 40, D-24098 Kiel, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't