Source:http://linkedlifedata.com/resource/pubmed/id/20025976
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2010-2-1
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| pubmed:databankReference | |
| pubmed:abstractText |
As a member of a newly discovered protein family, the suppressor of cytokine signalling 3 (SOCS-3) has been shown to regulate the responses of many immune cytokines in a negative auto-regulatory manner. The full-length cDNA of common carp SOCS-3 was 1603 bp and contained a 630 bp open reading frame (ORF) coding for a protein of 209 amino acids. Carp SOCS-3 molecule was well conserved especially in the SRC homology 2 (SH2) and the SOCS box. The kinase inhibitory region (KIR) and ESS domains, upstream of the SH2 domain, were conserved in carp SOCS-3, except for a specific insertion (PHRYK) in the KIR domain at the N-terminal region. Three conserved cysteine (Cys-102, 124 and 193) residues, and one additional cysteine (Cys-168) residue, were also found in carp SOCS-3. The 2015 bp genomic DNA of carp SOCS-3 contained two exons and one intron. Phylogenetic analysis showed that carp SOCS-3 sequence grouped with other known fish SOCS-3 sequences with zebrafish SOCS-3 as the closest neighbour. RT-PCR analysis showed that carp SOCS-3 was initially expressed at 4 h pf (post-fertilization) and gradually increased up to 4 w pf during embryogenesis. By RT-qPCR analysis, carp SOCS-3 gene was predominantly detected in gill, head kidney, thymus and skin, followed by spleen and peripheral blood, lower expression level was detected in kidney, intestine, liver and muscle; the SOCS-3 transcript was significantly increased in thymus, head kidney, spleen and intestine of GH (growth hormone)-transgenic carp; after SVCV (spring viraemia of carp virus) infection, the carp SOCS-3 transcript was significantly up-regulated in gill, intestine, thymus, spleen, head kidney and kidney tissues in a time-dependent manner. These results suggest that teleost SOCS-3 may play an active role in the modulation of viral-induced innate immune response and in preventing the overaction of some cytokines with viral stimulation.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
1095-9947
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| pubmed:author | |
| pubmed:copyrightInfo |
Crown Copyright 2009. Published by Elsevier Ltd. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
28
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
362-71
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| pubmed:meshHeading |
pubmed-meshheading:20025976-Amino Acid Sequence,
pubmed-meshheading:20025976-Animals,
pubmed-meshheading:20025976-Animals, Genetically Modified,
pubmed-meshheading:20025976-Base Sequence,
pubmed-meshheading:20025976-Carps,
pubmed-meshheading:20025976-Cloning, Molecular,
pubmed-meshheading:20025976-Embryonic Development,
pubmed-meshheading:20025976-Fish Diseases,
pubmed-meshheading:20025976-Gene Expression Profiling,
pubmed-meshheading:20025976-Gene Expression Regulation,
pubmed-meshheading:20025976-Growth Hormone,
pubmed-meshheading:20025976-Molecular Sequence Data,
pubmed-meshheading:20025976-Phylogeny,
pubmed-meshheading:20025976-Rhabdoviridae Infections,
pubmed-meshheading:20025976-Sequence Alignment,
pubmed-meshheading:20025976-Sequence Homology, Amino Acid,
pubmed-meshheading:20025976-Suppressor of Cytokine Signaling Proteins,
pubmed-meshheading:20025976-Vesiculovirus
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| pubmed:year |
2010
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| pubmed:articleTitle |
Cloning of common carp SOCS-3 gene and its expression during embryogenesis, GH-transgene and viral infection.
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| pubmed:affiliation |
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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