Source:http://linkedlifedata.com/resource/pubmed/id/20023063
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
Pt 11
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pubmed:dateCreated |
2010-11-4
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pubmed:databankReference | |
pubmed:abstractText |
Two halophilic archaea, strains RO1-4(T) and RO1-64, were isolated from a marine solar saltern in Jiangsu, China. Cells of the two strains were pleomorphic, motile, and stained Gram-negative. Colonies were red-pigmented. Strains RO1-4(T) and RO1-64 were able to grow at 25-55 °C (optimum 40-42 °C), at 2.1-5.1 M NaCl (optimum 3.9 M NaCl), at 0.05-0.7 M MgCl(2) (optimum 0.3 M MgCl(2)) and at pH 6.0-8.5 (optimum pH 7.0). Cells lyse in distilled water and the minimal NaCl concentration to prevent cell lysis is 12?% (w/v). On the basis of 16S rRNA gene sequence analysis, strains RO1-4(T) and RO1-64 were closely related to Halogeometricum borinquense PR3(T) (98.0 and 98.2?% similarity, respectively) and Halosarcina pallida BZ256(T) (97.8 and 97.9?%). The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two glycolipids (S-DGD-1 and DGD-1). The DNA G+C contents of strains RO1-4(T) and RO1-64 are 64.9 and 62.4 mol%, respectively. The DNA-DNA hybridization value between strains RO1-4(T) and RO1-64 was 83.0?% and both strains showed low DNA-DNA relatedness with Halogeometricum borinquense PR3(T) (42.5 and 50.1?% relatedness, respectively) and Halosarcina pallida BZ256(T) (37.6 and 42.1?% relatedness). It was concluded that strains RO1-4(T) and RO1-64 represent a novel species of the genus Halogeometricum, for which the name Halogeometricum rufum sp. nov. is proposed. The type strain is RO1-4(T) (=CGMCC 1.7736(T) =JCM 15770(T)).
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1466-5034
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
60
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2613-7
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pubmed:meshHeading |
pubmed-meshheading:20023063-DNA, Archaeal,
pubmed-meshheading:20023063-Halobacteriaceae,
pubmed-meshheading:20023063-Molecular Sequence Data,
pubmed-meshheading:20023063-Phylogeny,
pubmed-meshheading:20023063-RNA, Ribosomal, 16S,
pubmed-meshheading:20023063-Seawater,
pubmed-meshheading:20023063-Sodium Chloride
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pubmed:year |
2010
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pubmed:articleTitle |
Halogeometricum rufum sp. nov., a halophilic archaeon from a marine solar saltern, and emended description of the genus Halogeometricum.
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pubmed:affiliation |
School of Food and Biological Engineering, Jiangsu University, Zhenjiang, PR China. cuihenglin@sohu.com
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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