| pubmed-article:2002059 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2002059 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2002059 | lifeskim:mentions | umls-concept:C0229655 | lld:lifeskim |
| pubmed-article:2002059 | lifeskim:mentions | umls-concept:C0600531 | lld:lifeskim |
| pubmed-article:2002059 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:2002059 | lifeskim:mentions | umls-concept:C1333201 | lld:lifeskim |
| pubmed-article:2002059 | lifeskim:mentions | umls-concept:C1413043 | lld:lifeskim |
| pubmed-article:2002059 | lifeskim:mentions | umls-concept:C0332324 | lld:lifeskim |
| pubmed-article:2002059 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:2002059 | pubmed:dateCreated | 1991-4-17 | lld:pubmed |
| pubmed-article:2002059 | pubmed:abstractText | Human monoblast U937 cells contain a soluble phospholipase A2 (PLA2) that is activated over the range of 150-600 nM Ca2+ and is stable only at neutral pH. We have purified this PLA2 over 34,000-fold to near homogeneity using sequential ion exchange, hydrophobic interaction, and gel filtration chromatography steps. The protein has a Mr of approximately 100,000 (by sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and an isoelectric point of 5.1. Four lines of evidence indicate that this 100-kDa polypeptide represents the PLA2. (i) The intensity of staining of the 100-kDa protein was proportional to the degree of purification of PLA2 activity, (ii) the relative staining intensity of the 100-kDa protein precisely paralleled the elution profile of PLA2 activity during chromatography steps, (iii) the PLA2 activity recovered from a nondenaturing gel (greater than 60% of the total activity applied) coincided exactly with the major high molecular weight protein detected by silver staining, and (iv) monoclonal antibodies against the 100-kDa protein immunoprecipitated the PLA2. We conclude that the cytosolic PLA2 isolated from U937 cells represents a novel, high molecular weight PLA2 responding to physiological (intracellular) changes in Ca2+ concentration and therefore may play a critical role in cellular signal transduction processes and the biosynthesis of lipid mediators. | lld:pubmed |
| pubmed-article:2002059 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2002059 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2002059 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2002059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2002059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2002059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2002059 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2002059 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:2002059 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:2002059 | pubmed:author | pubmed-author:KramerR MRM | lld:pubmed |
| pubmed-article:2002059 | pubmed:author | pubmed-author:ManettaJJ | lld:pubmed |
| pubmed-article:2002059 | pubmed:author | pubmed-author:RobertsE FEF | lld:pubmed |
| pubmed-article:2002059 | pubmed:author | pubmed-author:PutnamJ EJE | lld:pubmed |
| pubmed-article:2002059 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2002059 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:2002059 | pubmed:volume | 266 | lld:pubmed |
| pubmed-article:2002059 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2002059 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2002059 | pubmed:pagination | 5268-72 | lld:pubmed |
| pubmed-article:2002059 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:2002059 | pubmed:meshHeading | pubmed-meshheading:2002059-... | lld:pubmed |
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| pubmed-article:2002059 | pubmed:meshHeading | pubmed-meshheading:2002059-... | lld:pubmed |
| pubmed-article:2002059 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:2002059 | pubmed:articleTitle | The Ca2(+)-sensitive cytosolic phospholipase A2 is a 100-kDa protein in human monoblast U937 cells. | lld:pubmed |
| pubmed-article:2002059 | pubmed:affiliation | Lilly Research Laboratories, Indianapolis, Indiana 46285. | lld:pubmed |
| pubmed-article:2002059 | pubmed:publicationType | Journal Article | lld:pubmed |
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