Linked Life Data

20015503

Source:http://linkedlifedata.com/resource/pubmed/id/20015503

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-1-28
pubmed:abstractText
A simple, sensitive and reliable analytical method for the rapid simultaneous determination of dexamethasone and betamethasone in milk by high performance liquid chromatography-negative electrospray ionization tandem mass spectrometry (HPLC-NESI-MS/MS) with isotope dilution was developed. Samples were directly purified through C(18) cartridge. Then the eluate was dried under nitrogen and residues were dissolved in mobile phase. Samples were analyzed by HPLC-MS/MS on a Hypercarb graphite column with a mixture of acetonitrile-water-formic acid as mobile phase. The samples were quantified using dexamethasone-D(4) as an internal standard. The procedure was validated according to the European Union regulation 2002/657/EC determining specificity, decision limit (CCalpha), detection capability (CCbeta), trueness, precision, linearity and stability. The method is demonstrated to be suitable for the determination of dexamethasone and betamethasone in milk. The total time required for the analysis of one sample was about 35 min.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1873-3778
pubmed:author
pubmed:copyrightInfo
2009 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
1217
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
411-4
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Rapid simultaneous determination of dexamethasone and betamethasone in milk by liquid chromatography tandem mass spectrometry with isotope dilution.
pubmed:affiliation
College of Agriculture, Hebei University of Engineering, Handan 056038, China.
pubmed:publicationType
Journal Article