|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
2010-6-3
|
|
pubmed:abstractText |
The time-resolved fluorescence technique based on melanin-concentrating hormone (MCH) receptor subtype-1 (MCH-1 receptor) binding assay was adopted to carry out a bioassay-guided fractionation of the methanol extract of Morus alba leaves. This fractionation and purification led to the isolation of two compounds identified as pheophorbide a methyl ester and 13(2)(S)-hydroxypheophorbide a methyl ester. These active pheophorbides exhibited potent inhibitory activity in binding of europium-labeled MCH to the human recombinant MCH-1 receptor (IC(50) value; 4.03 and 0.33 microM, respectively). Besides binding activity, the pheophorbides inhibited MCH-mediated extracellular signal-regulated kinase (ERK) phosphorylation in Chinese hamster ovary cells expressing human MCH-1 receptor. These results suggest that pheophorbide a methyl ester and 13(2)(S)-hydroxypheophorbide a methyl ester act as modulators of MCH-1 receptor and MCH-mediated ERK signaling.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
1099-1573
|
|
pubmed:author |
|
|
pubmed:copyrightInfo |
(c) 2009 John Wiley & Sons, Ltd.
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
24
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
919-23
|
|
pubmed:meshHeading |
pubmed-meshheading:20013814-Animals,
pubmed-meshheading:20013814-CHO Cells,
pubmed-meshheading:20013814-Chlorophyll,
pubmed-meshheading:20013814-Cricetinae,
pubmed-meshheading:20013814-Cricetulus,
pubmed-meshheading:20013814-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:20013814-Humans,
pubmed-meshheading:20013814-Molecular Structure,
pubmed-meshheading:20013814-Morus,
pubmed-meshheading:20013814-Phosphorylation,
pubmed-meshheading:20013814-Plant Extracts,
pubmed-meshheading:20013814-Plant Leaves,
pubmed-meshheading:20013814-Receptors, Pituitary Hormone,
pubmed-meshheading:20013814-Recombinant Proteins,
pubmed-meshheading:20013814-Signal Transduction
|
|
pubmed:year |
2010
|
|
pubmed:articleTitle |
Melanin-concentrating hormone-1 receptor binding activity of pheophorbides isolated from Morus alba leaves.
|
|
pubmed:affiliation |
Bio-Organic Science Division, Korea Research Institute of Chemical Technology, Yuseong, Daejeon, Republic of Korea.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
