Source:http://linkedlifedata.com/resource/pubmed/id/20010428
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2010-2-1
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| pubmed:abstractText |
Serotonin (5-hydroxytryptamine, 5-HT) is known to be a mitogenic factor in several malignancies. It elicits its mitogenic effect through a wide range of 5-HT receptor subtypes and several internal cellular transcription pathways. According to wide distribution of 5-HT3 and 5-HT4 receptors in the gastrointestinal tract, the main aim of this study was to investigate the effect of these receptor agonists and antagonists in a colorectal cell line. In cell culture, we investigated the effects of 5-HT, 5-HT3 and 5-HT4 receptor agonists and antagonists on proliferation of HT29 cells. We also tested apoptosis for the receptor antagonists with TUNEL apoptosis test. In addition, we assayed effects of 5-HT receptor antagonists on cell cycle kinetics with flow cytometery. Proliferation assay revealed that phenylbiguanide (a 5-HT3 receptor selective agonist) increased proliferation of HT29 cells significantly and Y25130 hydrochloride (a 5-HT3 receptor antagonist) had the opposite effect; but for 5-HT4 receptor antagonist, these effects were not significant. In addition, potent apoptotic and cell cycle arresting effect was found for a selective 5-HT3 receptor antagonist but we have not seen any significant effect for the 5-HT4 receptor antagonist. The findings of this study provide strong evidence for the potential role of 5-HT3 receptors in colorectal cancer.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antimitotic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Bicyclo Compounds, Heterocyclic,
http://linkedlifedata.com/resource/pubmed/chemical/Oxazines,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin 5-HT3 Receptor Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin 5-HT3 Receptor Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin Receptor Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/azasetron
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| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
1473-5709
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
19
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
138-43
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:20010428-Antimitotic Agents,
pubmed-meshheading:20010428-Apoptosis,
pubmed-meshheading:20010428-Bicyclo Compounds, Heterocyclic,
pubmed-meshheading:20010428-Blotting, Western,
pubmed-meshheading:20010428-Cell Cycle,
pubmed-meshheading:20010428-Cell Line, Tumor,
pubmed-meshheading:20010428-Cell Proliferation,
pubmed-meshheading:20010428-Colorectal Neoplasms,
pubmed-meshheading:20010428-Flow Cytometry,
pubmed-meshheading:20010428-Humans,
pubmed-meshheading:20010428-Microscopy, Fluorescence,
pubmed-meshheading:20010428-Oxazines,
pubmed-meshheading:20010428-Serotonin 5-HT3 Receptor Agonists,
pubmed-meshheading:20010428-Serotonin 5-HT3 Receptor Antagonists,
pubmed-meshheading:20010428-Serotonin Antagonists,
pubmed-meshheading:20010428-Serotonin Receptor Agonists,
pubmed-meshheading:20010428-Tumor Cells, Cultured
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| pubmed:year |
2010
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| pubmed:articleTitle |
Y25130 hydrochloride, a selective 5HT3 receptor antagonist has potent antimitogenic and apoptotic effect on HT29 colorectal cancer cell line.
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| pubmed:affiliation |
Department of Immunology, Tehran University of Medical Sciences, Tehran, Iran.
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| pubmed:publicationType |
Journal Article
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