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rdf:type |
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lifeskim:mentions |
umls-concept:C0026336,
umls-concept:C0085358,
umls-concept:C0087111,
umls-concept:C0205082,
umls-concept:C0205263,
umls-concept:C0272286,
umls-concept:C0332324,
umls-concept:C0591833,
umls-concept:C0871261,
umls-concept:C1332717,
umls-concept:C1413244,
umls-concept:C1704632,
umls-concept:C1706438,
umls-concept:C1706817,
umls-concept:C2698600,
umls-concept:C2911692
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pubmed:issue |
6
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pubmed:dateCreated |
2010-2-12
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pubmed:abstractText |
Immune thrombocytopenia (ITP) is a bleeding disorder characterized by antibody-opsonized platelets being prematurely destroyed in the spleen, although some patients with ITP may have a cell-mediated form of thrombocytopenia. Although several animal models of ITP have been developed, few mimic primary chronic ITP nor have any shown cell-mediated platelet destruction. To create this type of model, splenocytes from CD61 knockout mice immunized against CD61(+) platelets were transferred into severe combined immunodeficient (SCID) (CD61(+)) mouse recipients, and their platelet counts and phenotypes were observed. As few as 5 x 10(4) splenocytes induced a significant thrombocytopenia and bleeding mortality (80%) in recipients within 3 weeks after transfer. Depletion of lymphocyte subsets before transfer showed that the splenocyte's ability to induce thrombocytopenia and bleeding completely depended on CD4(+) T helper cells and that both CD19(+) B cell (antibody)- and CD8(+) T cell (cell)-mediated effector mechanisms were responsible. Treatment of the SCID mouse recipients with intravenous gamma-globulins raised platelet counts and completely prevented bleeding mortality induced by antibody-mediated effector mechanisms but did not affect cell-mediated disease. This novel model not only shows both antibody- and cell-mediated ITP and bleeding but also suggests that these 2 effector mechanisms have a differential response to therapy.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1528-0020
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pubmed:author |
pubmed-author:AslamRukhsanaR,
pubmed-author:ChenPingguoP,
pubmed-author:ChowLeolaL,
pubmed-author:CridlandNormanN,
pubmed-author:FreedmanJohnJ,
pubmed-author:GarveyM BernadetteMB,
pubmed-author:JungM YMY,
pubmed-author:KimMichaelM,
pubmed-author:LazarusAlan HAH,
pubmed-author:SahibKimK,
pubmed-author:SempleJohn WJW,
pubmed-author:SpeckEdwin RER,
pubmed-author:WebsterMichelle LeeML
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pubmed:issnType |
Electronic
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pubmed:day |
11
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pubmed:volume |
115
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1247-53
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pubmed:dateRevised |
2010-6-1
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pubmed:meshHeading |
pubmed-meshheading:20007808-Animals,
pubmed-meshheading:20007808-Antigens, CD19,
pubmed-meshheading:20007808-Blood Platelets,
pubmed-meshheading:20007808-CD8-Positive T-Lymphocytes,
pubmed-meshheading:20007808-Disease Models, Animal,
pubmed-meshheading:20007808-Female,
pubmed-meshheading:20007808-Flow Cytometry,
pubmed-meshheading:20007808-Immunoglobulins, Intravenous,
pubmed-meshheading:20007808-Integrin beta3,
pubmed-meshheading:20007808-Lymphocyte Depletion,
pubmed-meshheading:20007808-Megakaryocytes,
pubmed-meshheading:20007808-Mice,
pubmed-meshheading:20007808-Mice, Inbred BALB C,
pubmed-meshheading:20007808-Mice, Inbred C57BL,
pubmed-meshheading:20007808-Mice, Knockout,
pubmed-meshheading:20007808-Mice, SCID,
pubmed-meshheading:20007808-Platelet Count,
pubmed-meshheading:20007808-Purpura, Thrombocytopenic, Idiopathic,
pubmed-meshheading:20007808-Spleen,
pubmed-meshheading:20007808-T-Lymphocytes, Helper-Inducer
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pubmed:year |
2010
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pubmed:articleTitle |
A murine model of severe immune thrombocytopenia is induced by antibody- and CD8+ T cell-mediated responses that are differentially sensitive to therapy.
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pubmed:affiliation |
Toronto Platelet Immunobiology Group, Toronto, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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