Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-1-25
pubmed:abstractText
Pre-mRNAs of the influenza A virus M and NS genes are poorly spliced in virus-infected cells. By contrast, in influenza C virus-infected cells, the predominant transcript from the M gene is spliced mRNA. The present study was performed to investigate the mechanism by which influenza C virus M gene-specific mRNA (M mRNA) is readily spliced. The ratio of M1 encoded by a spliced M mRNA to CM2 encoded by an unspliced M mRNA in influenza C virus-infected cells was about 10 times larger than that in M gene-transfected cells, suggesting that a viral protein(s) other than M gene translational products facilitates viral mRNA splicing. RNase protection assays showed that the splicing of M mRNA in infected cells was much higher than that in M gene-transfected cells. The unspliced and spliced mRNAs of the influenza C virus NS gene encode two nonstructural (NS) proteins, NS1(C/NS1) and NS2(C/NS2), respectively. The introduction of premature translational termination into the NS gene, which blocked the synthesis of the C/NS1 and C/NS2 proteins, drastically reduced the splicing of NS mRNA, raising the possibility that C/NS1 or C/NS2 enhances viral mRNA splicing. The splicing of influenza C virus M mRNA was increased by coexpression of C/NS1, whereas it was reduced by coexpression of the influenza A virus NS1 protein (A/NS1). The splicing of influenza A virus M mRNA was also increased by coexpression of C/NS1, though it was inhibited by that of A/NS1. These results suggest that influenza C virus NS1, but not A/NS1, can upregulate viral mRNA splicing.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-10900030, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-11462009, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-1388016, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-14689274, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-15218173, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-1532050, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-1626420, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-17488845, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-17537859, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-17622634, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-1833874, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-19130168, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-1985199, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-1990058, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-2827008, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-3404579, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-3799062, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-6246509, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-6314642, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-6945577, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-7097250, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-7507186, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-7541537, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-7958859, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-7996141, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-8128618, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-8289394, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-8313914, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-8393074, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-8666660, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-8895585, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-9060633, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-9747730, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-9789071, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-9847305, http://linkedlifedata.com/resource/pubmed/commentcorrection/20007279-9918879
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1098-5514
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1957-66
pubmed:dateRevised
2010-9-27
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Influenza C virus NS1 protein upregulates the splicing of viral mRNAs.
pubmed:affiliation
Department of Infectious Diseases, Yamagata University School of Medicine, 2-2-2 Iida-Nishi, Yamagata 990-9585, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't