| pubmed-article:20006615 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20006615 | lifeskim:mentions | umls-concept:C0024432 | lld:lifeskim |
| pubmed-article:20006615 | lifeskim:mentions | umls-concept:C1099354 | lld:lifeskim |
| pubmed-article:20006615 | lifeskim:mentions | umls-concept:C0011209 | lld:lifeskim |
| pubmed-article:20006615 | lifeskim:mentions | umls-concept:C0206691 | lld:lifeskim |
| pubmed-article:20006615 | lifeskim:mentions | umls-concept:C0591833 | lld:lifeskim |
| pubmed-article:20006615 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:20006615 | pubmed:dateCreated | 2010-2-17 | lld:pubmed |
| pubmed-article:20006615 | pubmed:abstractText | Selective gene silencing by RNA interference (RNAi) is a valuable tool for the targeted manipulation of the development and/or function of cells. Using a fluorescein-labeled non-silencing siRNA duplex, we established a protocol for the electroporation of primary mouse macrophages which routinely yielded >95% transfected cells. Electroporation of siRNAs directed against MAPK1 and CD86 led to an efficient knock-down of cellular protein in bone marrow-derived mouse macrophages (BM-Mphi). Importantly, the electroporation procedure did not impair the viability of BM-Mphi, their ability to ingest or degrade E. coli or their capacity to express iNOS mRNA, to produce NO or to upregulate TNF and IL-6 mRNA in response to inflammatory stimuli such as LPS. Therefore, we propose that electroporation of silencing siRNAs into murine BM-Mphi is a highly efficient method to manipulate gene expression of BM-Mphi that does not cause toxicity or a non-specific alteration of macrophage biology. | lld:pubmed |
| pubmed-article:20006615 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20006615 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20006615 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20006615 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20006615 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20006615 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20006615 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20006615 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20006615 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20006615 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20006615 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20006615 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20006615 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20006615 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20006615 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20006615 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:20006615 | pubmed:issn | 1872-7905 | lld:pubmed |
| pubmed-article:20006615 | pubmed:author | pubmed-author:BogdanChristi... | lld:pubmed |
| pubmed-article:20006615 | pubmed:author | pubmed-author:CastiglioneKi... | lld:pubmed |
| pubmed-article:20006615 | pubmed:author | pubmed-author:HenselMichael... | lld:pubmed |
| pubmed-article:20006615 | pubmed:author | pubmed-author:SchleicherUlr... | lld:pubmed |
| pubmed-article:20006615 | pubmed:author | pubmed-author:JantschJonath... | lld:pubmed |
| pubmed-article:20006615 | pubmed:author | pubmed-author:WieseMelanieM | lld:pubmed |
| pubmed-article:20006615 | pubmed:copyrightInfo | 2009 Elsevier B.V. All rights reserved. | lld:pubmed |
| pubmed-article:20006615 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20006615 | pubmed:day | 28 | lld:pubmed |
| pubmed-article:20006615 | pubmed:volume | 353 | lld:pubmed |
| pubmed-article:20006615 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20006615 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20006615 | pubmed:pagination | 102-10 | lld:pubmed |
| pubmed-article:20006615 | pubmed:meshHeading | pubmed-meshheading:20006615... | lld:pubmed |
| pubmed-article:20006615 | pubmed:meshHeading | pubmed-meshheading:20006615... | lld:pubmed |
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| pubmed-article:20006615 | pubmed:meshHeading | pubmed-meshheading:20006615... | lld:pubmed |
| pubmed-article:20006615 | pubmed:meshHeading | pubmed-meshheading:20006615... | lld:pubmed |
| pubmed-article:20006615 | pubmed:meshHeading | pubmed-meshheading:20006615... | lld:pubmed |
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| pubmed-article:20006615 | pubmed:meshHeading | pubmed-meshheading:20006615... | lld:pubmed |
| pubmed-article:20006615 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20006615 | pubmed:articleTitle | Small interfering RNA (siRNA) delivery into murine bone marrow-derived macrophages by electroporation. | lld:pubmed |
| pubmed-article:20006615 | pubmed:affiliation | Microbiology Institute-Clinical Microbiology, Immunology and Hygiene, University Clinic Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, Erlangen, Germany. | lld:pubmed |
| pubmed-article:20006615 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20006615 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:20006615 | lld:pubmed |
