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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2010-2-17
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pubmed:abstractText |
Selective gene silencing by RNA interference (RNAi) is a valuable tool for the targeted manipulation of the development and/or function of cells. Using a fluorescein-labeled non-silencing siRNA duplex, we established a protocol for the electroporation of primary mouse macrophages which routinely yielded >95% transfected cells. Electroporation of siRNAs directed against MAPK1 and CD86 led to an efficient knock-down of cellular protein in bone marrow-derived mouse macrophages (BM-Mphi). Importantly, the electroporation procedure did not impair the viability of BM-Mphi, their ability to ingest or degrade E. coli or their capacity to express iNOS mRNA, to produce NO or to upregulate TNF and IL-6 mRNA in response to inflammatory stimuli such as LPS. Therefore, we propose that electroporation of silencing siRNAs into murine BM-Mphi is a highly efficient method to manipulate gene expression of BM-Mphi that does not cause toxicity or a non-specific alteration of macrophage biology.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD86,
http://linkedlifedata.com/resource/pubmed/chemical/Cd86 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Mapk1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Nos2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1872-7905
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pubmed:author |
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pubmed:copyrightInfo |
2009 Elsevier B.V. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
28
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pubmed:volume |
353
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
102-10
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pubmed:meshHeading |
pubmed-meshheading:20006615-Animals,
pubmed-meshheading:20006615-Antigens, CD86,
pubmed-meshheading:20006615-Cell Survival,
pubmed-meshheading:20006615-Cells, Cultured,
pubmed-meshheading:20006615-Electroporation,
pubmed-meshheading:20006615-Female,
pubmed-meshheading:20006615-Interleukin-6,
pubmed-meshheading:20006615-Lipopolysaccharides,
pubmed-meshheading:20006615-Macrophages,
pubmed-meshheading:20006615-Mice,
pubmed-meshheading:20006615-Mice, Inbred C57BL,
pubmed-meshheading:20006615-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:20006615-Nitric Oxide Synthase Type II,
pubmed-meshheading:20006615-Phagocytosis,
pubmed-meshheading:20006615-RNA, Messenger,
pubmed-meshheading:20006615-RNA, Small Interfering,
pubmed-meshheading:20006615-RNA Interference,
pubmed-meshheading:20006615-Time Factors,
pubmed-meshheading:20006615-Transfection,
pubmed-meshheading:20006615-Tumor Necrosis Factor-alpha
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pubmed:year |
2010
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pubmed:articleTitle |
Small interfering RNA (siRNA) delivery into murine bone marrow-derived macrophages by electroporation.
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pubmed:affiliation |
Microbiology Institute-Clinical Microbiology, Immunology and Hygiene, University Clinic Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, Erlangen, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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