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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2010-4-19
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pubmed:abstractText |
Dendritic cells (DCs) are applied worldwide in several clinical studies of immune therapy of malignancies, autoimmune diseases, and transplantations. Most legislative bodies are demanding high standards for cultivation and transduction of cells. Closed-cell cultivating systems like cell culture bags would simplify and greatly improve the ability to reach these cultivation standards. We investigated if a new polyolefin cell culture bag enables maturation and adenoviral modification of human DCs in a closed system and compare the results with standard polystyrene flasks.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1537-2995
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pubmed:author |
pubmed-author:DittmarKurt E JKE,
pubmed-author:GarritsenHenk S PHS,
pubmed-author:GeffersRobertR,
pubmed-author:HannigHorstH,
pubmed-author:LindenmaierWernerW,
pubmed-author:MackeLarsL,
pubmed-author:MeyringWilhelmW,
pubmed-author:PägelowUteU,
pubmed-author:PiechaczekChristophC,
pubmed-author:RohdeManfredM,
pubmed-author:WörmannBernhardB
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pubmed:issnType |
Electronic
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pubmed:volume |
50
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
843-55
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pubmed:meshHeading |
pubmed-meshheading:20003054-Adult,
pubmed-meshheading:20003054-Antigens, CD,
pubmed-meshheading:20003054-Antigens, CD14,
pubmed-meshheading:20003054-Antigens, Surface,
pubmed-meshheading:20003054-Cell Aging,
pubmed-meshheading:20003054-Cell Count,
pubmed-meshheading:20003054-Cell Culture Techniques,
pubmed-meshheading:20003054-Cell Division,
pubmed-meshheading:20003054-Child,
pubmed-meshheading:20003054-Dendritic Cells,
pubmed-meshheading:20003054-Female,
pubmed-meshheading:20003054-Gene Transfer Techniques,
pubmed-meshheading:20003054-Humans,
pubmed-meshheading:20003054-Leukapheresis,
pubmed-meshheading:20003054-Leukocytes,
pubmed-meshheading:20003054-Male,
pubmed-meshheading:20003054-Microscopy, Electron, Scanning,
pubmed-meshheading:20003054-Middle Aged,
pubmed-meshheading:20003054-Oligonucleotide Array Sequence Analysis
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pubmed:year |
2010
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pubmed:articleTitle |
Evaluating maturation and genetic modification of human dendritic cells in a new polyolefin cell culture bag system.
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pubmed:affiliation |
Department of Molecular Biotechnology, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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