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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2010-2-9
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pubmed:abstractText |
3,4-Methylenedioxymethamphetamine (MDMA; 'Ecstasy') is a popular recreational drug used worldwide. This study aimed to determine the effects of this compound on the expression of nerve terminal serotonergic markers in rats. Experiment 1 investigated MDMA-induced changes in levels of the serotonin transporter (SERT) and the vesicular monoamine transporter 2 (VMAT-2) in the hippocampus, a region with sparse dopaminergic innervation, after lesioning noradrenergic input with N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP-4). Adult male Sprague-Dawley rats were administered 100 mg/kg DSP-4 or saline 1 week prior to either an MDMA (10 mg/kg x 4) or saline binge. Two weeks following the binge treatment, the DSP-4/MDMA group unexpectedly showed little change in hippocampal VMAT-2 protein expression compared with DSP-4/Saline controls, despite large reductions in SERT levels in all regions examined in the MDMA-treated animals. Furthermore, animals treated with binge MDMA (Experiment 2) showed a striking decrease in SERT gene expression (and a lesser effect on VMAT-2) measured by quantitative RT-PCR in pooled dorsal and median raphe tissue punches, when compared with saline-treated controls. These results demonstrate that MDMA causes substantial regulatory changes in the expression of serotonergic markers, thus questioning the need to invoke distal axotomy as an explanation of MDMA-related serotonergic deficits.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5,7-Dihydroxytryptamine,
http://linkedlifedata.com/resource/pubmed/chemical/Benzylamines,
http://linkedlifedata.com/resource/pubmed/chemical/Central Nervous System Stimulants,
http://linkedlifedata.com/resource/pubmed/chemical/DSP 4,
http://linkedlifedata.com/resource/pubmed/chemical/Hallucinogens,
http://linkedlifedata.com/resource/pubmed/chemical/Methamphetamine,
http://linkedlifedata.com/resource/pubmed/chemical/N-Methyl-3,4-methylenedioxyamphetami...,
http://linkedlifedata.com/resource/pubmed/chemical/Neurotransmitter Uptake Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin Plasma Membrane...,
http://linkedlifedata.com/resource/pubmed/chemical/Slc18a2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Vesicular Monoamine Transport...
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1471-4159
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
112
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
951-62
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pubmed:meshHeading |
pubmed-meshheading:20002520-5,7-Dihydroxytryptamine,
pubmed-meshheading:20002520-Animals,
pubmed-meshheading:20002520-Area Under Curve,
pubmed-meshheading:20002520-Benzylamines,
pubmed-meshheading:20002520-Brain,
pubmed-meshheading:20002520-Central Nervous System Stimulants,
pubmed-meshheading:20002520-Disease Models, Animal,
pubmed-meshheading:20002520-Female,
pubmed-meshheading:20002520-Gene Expression,
pubmed-meshheading:20002520-Hallucinogens,
pubmed-meshheading:20002520-Male,
pubmed-meshheading:20002520-Methamphetamine,
pubmed-meshheading:20002520-N-Methyl-3,4-methylenedioxyamphetamine,
pubmed-meshheading:20002520-Neurotoxicity Syndromes,
pubmed-meshheading:20002520-Neurotransmitter Uptake Inhibitors,
pubmed-meshheading:20002520-Pregnancy,
pubmed-meshheading:20002520-Protein Binding,
pubmed-meshheading:20002520-Rats,
pubmed-meshheading:20002520-Rats, Sprague-Dawley,
pubmed-meshheading:20002520-Serotonin,
pubmed-meshheading:20002520-Serotonin Plasma Membrane Transport Proteins,
pubmed-meshheading:20002520-Synaptosomes,
pubmed-meshheading:20002520-Time Factors,
pubmed-meshheading:20002520-Vesicular Monoamine Transport Proteins
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pubmed:year |
2010
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pubmed:articleTitle |
Effects of 3,4-methylenedioxymethamphetamine (MDMA) on serotonin transporter and vesicular monoamine transporter 2 protein and gene expression in rats: implications for MDMA neurotoxicity.
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pubmed:affiliation |
Neuroscience and Behavior Program, University of Massachusetts, Amherst, Massachusetts, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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