| pubmed-article:20000848 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20000848 | lifeskim:mentions | umls-concept:C0237401 | lld:lifeskim |
| pubmed-article:20000848 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:20000848 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:20000848 | lifeskim:mentions | umls-concept:C0332282 | lld:lifeskim |
| pubmed-article:20000848 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:20000848 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:20000848 | pubmed:dateCreated | 2010-1-14 | lld:pubmed |
| pubmed-article:20000848 | pubmed:abstractText | The frequencies of antigen-specific CD4+ T cells in samples of human tissue have been difficult to determine accurately ex vivo, particularly for autoimmune diseases such as multiple sclerosis or type 1 diabetes. Conventional approaches involve the expansion of primary T cells in vitro to increase the numbers of cells, and a subsequent assessment of the frequencies of antigen-specific T cells in the expanded population by limiting dilution or by using fluorescently labeled tetramers of peptide-loaded major histocompatibility complex (MHC) receptors. Here we describe an alternative approach that uses arrays of subnanoliter wells coated with recombinant peptide-loaded MHC class II monomers to isolate and stimulate individual CD4+ T cells in an antigen-specific manner. In these experiments, activation was monitored using microengraving to capture two cytokines (IFNgamma and IL-17) released from single cells. This new method should enable direct enumeration of antigen-specific CD4+ T cells ex vivo from clinical samples. | lld:pubmed |
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| pubmed-article:20000848 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20000848 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20000848 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20000848 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20000848 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20000848 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20000848 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20000848 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20000848 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20000848 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:20000848 | pubmed:issn | 1520-6882 | lld:pubmed |
| pubmed-article:20000848 | pubmed:author | pubmed-author:AdiBB | lld:pubmed |
| pubmed-article:20000848 | pubmed:author | pubmed-author:NepomGerald... | lld:pubmed |
| pubmed-article:20000848 | pubmed:author | pubmed-author:LoveJ... | lld:pubmed |
| pubmed-article:20000848 | pubmed:author | pubmed-author:HaflerDavid... | lld:pubmed |
| pubmed-article:20000848 | pubmed:author | pubmed-author:KentSally CSC | lld:pubmed |
| pubmed-article:20000848 | pubmed:author | pubmed-author:NilssonBjörnB | lld:pubmed |
| pubmed-article:20000848 | pubmed:author | pubmed-author:HanQingQ | lld:pubmed |
| pubmed-article:20000848 | pubmed:author | pubmed-author:BradshawEliza... | lld:pubmed |
| pubmed-article:20000848 | pubmed:author | pubmed-author:RaddassiKhadi... | lld:pubmed |
| pubmed-article:20000848 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20000848 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:20000848 | pubmed:volume | 82 | lld:pubmed |
| pubmed-article:20000848 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20000848 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20000848 | pubmed:pagination | 473-7 | lld:pubmed |
| pubmed-article:20000848 | pubmed:dateRevised | 2011-9-26 | lld:pubmed |
| pubmed-article:20000848 | pubmed:meshHeading | pubmed-meshheading:20000848... | lld:pubmed |
| pubmed-article:20000848 | pubmed:meshHeading | pubmed-meshheading:20000848... | lld:pubmed |
| pubmed-article:20000848 | pubmed:meshHeading | pubmed-meshheading:20000848... | lld:pubmed |
| pubmed-article:20000848 | pubmed:meshHeading | pubmed-meshheading:20000848... | lld:pubmed |
| pubmed-article:20000848 | pubmed:meshHeading | pubmed-meshheading:20000848... | lld:pubmed |
| pubmed-article:20000848 | pubmed:meshHeading | pubmed-meshheading:20000848... | lld:pubmed |
| pubmed-article:20000848 | pubmed:meshHeading | pubmed-meshheading:20000848... | lld:pubmed |
| pubmed-article:20000848 | pubmed:meshHeading | pubmed-meshheading:20000848... | lld:pubmed |
| pubmed-article:20000848 | pubmed:meshHeading | pubmed-meshheading:20000848... | lld:pubmed |
| pubmed-article:20000848 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20000848 | pubmed:articleTitle | On-chip activation and subsequent detection of individual antigen-specific T cells. | lld:pubmed |
| pubmed-article:20000848 | pubmed:affiliation | Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA. | lld:pubmed |
| pubmed-article:20000848 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20000848 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:20000848 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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