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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2010-1-14
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pubmed:abstractText |
The frequencies of antigen-specific CD4+ T cells in samples of human tissue have been difficult to determine accurately ex vivo, particularly for autoimmune diseases such as multiple sclerosis or type 1 diabetes. Conventional approaches involve the expansion of primary T cells in vitro to increase the numbers of cells, and a subsequent assessment of the frequencies of antigen-specific T cells in the expanded population by limiting dilution or by using fluorescently labeled tetramers of peptide-loaded major histocompatibility complex (MHC) receptors. Here we describe an alternative approach that uses arrays of subnanoliter wells coated with recombinant peptide-loaded MHC class II monomers to isolate and stimulate individual CD4+ T cells in an antigen-specific manner. In these experiments, activation was monitored using microengraving to capture two cytokines (IFNgamma and IL-17) released from single cells. This new method should enable direct enumeration of antigen-specific CD4+ T cells ex vivo from clinical samples.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-10606632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-10903775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-11005833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-11748273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-12093009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-12731068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-14647479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-15047201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-15616737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-16030184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-16617008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-16871208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-1694881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-1694970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-1700423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-17186633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-17910063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-17972305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-18675591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-18813394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-19004776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-19012320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-19363473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-19482555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-19485404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-19684583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-19739655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-2465550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20000848-9151896
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1520-6882
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
473-7
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
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pubmed:year |
2010
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pubmed:articleTitle |
On-chip activation and subsequent detection of individual antigen-specific T cells.
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pubmed:affiliation |
Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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