Linked Life Data

1999763

Source:http://linkedlifedata.com/resource/pubmed/id/1999763

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1991-4-10
pubmed:abstractText
A microtiter plate was coated with cell nuclei from HEp-2 cells, and the stabilized nuclei were fixed with acetone for an enzyme-linked immunosorbent assay (ELISA). Autoantibodies against nuclear antigens were detected from the sera of patients with various systemic rheumatic disease but not from healthy individuals by means of the nucleus ELISA procedure. Ninety-one percent of antinuclear antibody (ANA)-positive sera as demonstrated by immunofluorescence (IF) test were also judged as positive for ANAs by the nucleus ELISA and 5% of them as pseudo-positive. Patient's sera with homogeneous and fine-speckled IF patterns displayed the highest ELISA titers. A large portion of ss-A/Ro antigen is localized in the cytoplasm; therefore, anti-ss-A/Ro antibody was hardly detected by nucleus ELISA. The population of ANAs detectable by nucleus ELISA included anti-ss-B/La, anti-DNA, anti-histone, anti-Sm, anti-RNP, and anti-scl-70.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0887-8013
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
49-53
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Detection of anti-nuclear antibodies from patients with systemic rheumatic diseases by ELISA using HEp-2 cell nuclei.
pubmed:affiliation
Laboratory for Immunology, Hoechst Japan Limited, Saitama, Japan.
pubmed:publicationType
Journal Article, Comparative Study