19997187

Source:http://linkedlifedata.com/resource/pubmed/id/19997187

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0007634, umls-concept:C0205171, umls-concept:C0205266, umls-concept:C0596837, umls-concept:C1523867
pubmed:issue	22
pubmed:dateCreated	2009-12-9
pubmed:abstractText	This report presents a model-independent method of assessing contributions to the light scattering from individual organelles in single intact cells. We first measure the 3D index map of a living cell, and then modify the map in such a way so as to eliminate contrast due to a particular intracellular organelle. By calculating and comparing the light scattering distributions calculated from the original and modified index maps using the Rytov approximation, we extract the light scattering contribution from the particular organelle of interest. The relative contributions of the nucleus and nucleolus to the scattering of the entire cell are thus determined, and the applicability of the homogeneous spherical model to non-spherical and heterogeneous organelles in forward scattering is evaluated.
pubmed:grant	http://linkedlifedata.com/resource/pubmed/grant/P41 RR002594-24, http://linkedlifedata.com/resource/pubmed/grant/P41 RR002594-246076, http://linkedlifedata.com/resource/pubmed/grant/P41-RR02594-18
pubmed:commentsCorrections	http://linkedlifedata.com/resource/pubmed/commentcorrection/19997187-11916880, http://linkedlifedata.com/resource/pubmed/commentcorrection/19997187-12175287, http://linkedlifedata.com/resource/pubmed/commentcorrection/19997187-12839941, http://linkedlifedata.com/resource/pubmed/commentcorrection/19997187-13054689, http://linkedlifedata.com/resource/pubmed/commentcorrection/19997187-16196346, http://linkedlifedata.com/resource/pubmed/commentcorrection/19997187-17041654, http://linkedlifedata.com/resource/pubmed/commentcorrection/19997187-17694065, http://linkedlifedata.com/resource/pubmed/commentcorrection/19997187-18628809, http://linkedlifedata.com/resource/pubmed/commentcorrection/19997187-19129896, http://linkedlifedata.com/resource/pubmed/commentcorrection/19997187-19701437, http://linkedlifedata.com/resource/pubmed/commentcorrection/19997187-4134589
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/101137103
pubmed:citationSubset	IM
pubmed:status	MEDLINE
pubmed:month	Oct
pubmed:issn	1094-4087
pubmed:author	pubmed-author:BadizadeganKamranK, pubmed-author:ChoiWonshikW, pubmed-author:DasariRamachandra RRR, pubmed-author:FeldMichael SMS, pubmed-author:KalashnikovMaximM, pubmed-author:SungYongjinY, pubmed-author:YuChung-ChiehCC
pubmed:issnType	Electronic
pubmed:day	26
pubmed:volume	17
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	19674-81
pubmed:dateRevised	2010-9-28
pubmed:meshHeading	pubmed-meshheading:19997187-Computer Simulation, pubmed-meshheading:19997187-Light, pubmed-meshheading:19997187-Models, Biological, pubmed-meshheading:19997187-Nephelometry and Turbidimetry, pubmed-meshheading:19997187-Organelles, pubmed-meshheading:19997187-Scattering, Radiation
pubmed:year	2009
pubmed:articleTitle	Assessing light scattering of intracellular organelles in single intact living cells.
pubmed:affiliation	GR Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
pubmed:publicationType	Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural