Source:http://linkedlifedata.com/resource/pubmed/id/19995580
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2010-2-1
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pubmed:abstractText |
Detection of microbial DNA in clinical samples by real-time PCR requires a universal extraction method with equally efficient lysis of cell walls of all possible microorganisms. We demonstrated that physical disruption by bead-beating with 0.1mm beads in combination with MagNA Pure DNA III extraction enhances microbial lysis of diverse Gram-positive microorganisms and may be used to optimize DNA extraction protocols in routine clinical diagnostics.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1872-8359
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pubmed:author | |
pubmed:copyrightInfo |
Copyright 2009 Elsevier B.V. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
80
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
209-11
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pubmed:meshHeading |
pubmed-meshheading:19995580-Bacteriolysis,
pubmed-meshheading:19995580-Clinical Laboratory Techniques,
pubmed-meshheading:19995580-DNA, Bacterial,
pubmed-meshheading:19995580-Gram-Positive Bacteria,
pubmed-meshheading:19995580-Gram-Positive Bacterial Infections,
pubmed-meshheading:19995580-Microspheres,
pubmed-meshheading:19995580-Molecular Diagnostic Techniques
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pubmed:year |
2010
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pubmed:articleTitle |
Improved detection of microbial DNA after bead-beating before DNA isolation.
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pubmed:affiliation |
Department of Research & Development, Laboratory for Infectious Diseases Groningen, The Netherlands.
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pubmed:publicationType |
Journal Article
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