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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1991-4-4
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pubmed:abstractText |
By using a series of overlapping synthetic peptides that cover more than 75% of the amino acid sequence of the major surface glycoprotein (gp63) from Leishmania major, 11 T-cell epitopes in CBA and BALB/c mice have been identified. Six of the peptides were recognized by T cells of CBA mice recovered from L. major infection, while one was recognized by the T cells from BALB/c mice recovered from the infection following sublethal doses of gamma-irradiation. Lymph node cells from mice immunized with the peptides also responded to a number of the same peptides (seven in CBA and one in BALB/c). Peptide p10-28 induced proliferative T-cell responses in both CBA and BALB/c mice. Five of the peptides (p10-28, p22-40, p289-309, p459-471 and p467-482) induced vigorous T-cell response in CBA mice but were not recognized by T cells from recovered mice. Four other peptides (p321-336, p364-476, p372-385 and p378-396) were recognized by T cells from recovered CBA mice but could not induce a T-cell response in normal CBA mice. Three peptides (p146-171, p289-309 and p395-414) were both able to induce a T-cell response and were recognized by T cells from recovered mice. However, only two peptides (p146-171 and p467-482) were able to activate T cells, which also recognized epitopes expressed by antigen-presenting cells infected with promastigotes. T cells induced by p146-171 and p467-171 or a mixture of these two peptides were mainly CD4+ and produced interleukin (IL-2) and interferon-gamma (IFN-gamma) but not IL-4 upon antigen stimulation in vitro. These two peptides also induced a classical delayed type hypersensitivity (DTH) response in CBA mice. Furthermore, CBA mice immunized with a mixture of the two peptides in Coryne parvum or entrapped in liposomes induced significant resistance against L. major infection. The implications of these results in terms of a synthetic vaccine against leishmaniasis and the mechanism of the induction of Th1 and Th2 cells are discussed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-1261086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-15462939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-2144549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-2146362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-2320059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-2435793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-2521244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-2525175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-2568842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-2608099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-2708373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-2786033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-2903212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-3160786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-3257774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-3346625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-3495599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-3862105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-3950420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-6150440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-6870673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-7119442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1997399-7179418
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
72
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1997399-Amino Acid Sequence,
pubmed-meshheading:1997399-Animals,
pubmed-meshheading:1997399-Antigens, Protozoan,
pubmed-meshheading:1997399-Host-Parasite Interactions,
pubmed-meshheading:1997399-Hypersensitivity, Delayed,
pubmed-meshheading:1997399-Immunity, Active,
pubmed-meshheading:1997399-Leishmania tropica,
pubmed-meshheading:1997399-Leishmaniasis,
pubmed-meshheading:1997399-Lymphokines,
pubmed-meshheading:1997399-Metalloendopeptidases,
pubmed-meshheading:1997399-Mice,
pubmed-meshheading:1997399-Mice, Inbred BALB C,
pubmed-meshheading:1997399-Mice, Inbred CBA,
pubmed-meshheading:1997399-Molecular Sequence Data,
pubmed-meshheading:1997399-Phenotype,
pubmed-meshheading:1997399-Protozoan Proteins,
pubmed-meshheading:1997399-T-Lymphocytes,
pubmed-meshheading:1997399-Vaccines, Synthetic
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pubmed:year |
1991
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pubmed:articleTitle |
Identification and characterization of host-protective T-cell epitopes of a major surface glycoprotein (gp63) from Leishmania major.
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pubmed:affiliation |
Department of Experimental Immunobiology, Wellcome Biotech, Beckenham, Kent, U.K.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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