Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1991-3-27
pubmed:abstractText
The 52 kDa transformation-sensitive protein p52 was previously identified as a major substrate-associated component of normal rat kidney (NRK) fibroblasts [Higgins & Ryan (1989) Biochem. J. 257, 173-182]. p52 selectively localized to cellular fractions enriched in substrate focal-contact sites and associated ventral undersurface elements. Rapid attachment/spreading of NRK cells on to prepared p52 matrices and inhibition of fibroblast spreading by antibodies to p52 indicated that this protein participates in shape determination or cell-to-substrate adhesion. NRK cells transformed with Kirsten murine sarcoma virus (KiMSV), with a temperature-sensitive mutant (ts-371 KiMSV) and maintained at the permissive temperature, or with the cloned EJrasval.12 oncogene, exhibited down-regulated accumulation of p52 in the ventral undersurface region. Immunochemical, lectin-affinity and electrophoretic analyses indicated that p52 shares considerable sequence similarity with plasminogen-activator inhibitor type-1, which is consistent with its subcellular localization and likely morphoregulatory activity. The marked down-regulation of p52 expression seen in four different ras-mediated transformation systems, its induction prior to butyrate-induced morphological reorganization in KiMSV-transformed cells, and the morphological consequences of exogenously added p52 or p52 antibodies on NRK fibroblasts suggest that this protein probably functions in cell-shape regulation. Abrogation of p52 matrix accumulation typically seen in ras transformants may contribute, therefore, to the aberrant cytoarchitecture characteristic of malignant fibroblasts.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-185074, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-225570, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-2289622, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-2298740, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-2332454, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-2434480, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-2459123, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-2469534, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-2497739, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-2498314, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-2521461, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-2546834, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-2547991, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-2613765, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-2691518, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-2715193, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-2831462, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-2844835, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-2916655, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-2920008, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-2930999, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-3023389, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-3044445, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-3104349, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-3121597, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-3121634, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-3123496, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-3139666, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-3141060, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-3262618, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-326414, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-3379954, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-3394802, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-3543023, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-3670292, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-3675551, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-3803451, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-3803452, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-3921554, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-6271758, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-6286138, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-6351729, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-6641816, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-6760990, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-7204498, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-7274345, http://linkedlifedata.com/resource/pubmed/commentcorrection/1996963-779852
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
273 ( Pt 3)
pubmed:geneSymbol
ras
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
651-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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