Linked Life Data

19969347

Source:http://linkedlifedata.com/resource/pubmed/id/19969347

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2010-1-29
pubmed:abstractText
Poly(acrylic acid) was decorated onto Fe(3)O(4) resulting in a highly water-soluble superparamagnetic iron oxide. The Poly(acrylic acid) iron oxide (PAAIO) complexes possess specific magnetic properties in the presence of an external magnetic field and are attractive contrast agents for magnetic resonance imaging (MRI). The free carboxylic groups of PAAIO exposed on the surface allow for covalent attachment of a fluorescent dye, Rhodamine 123 (Rh123) to form PAAIO-Rh123, which permits applications in fluorescence imaging. PAAIO-Rh123 is therefore a dual-modality molecular probe. In order to endow specific properties to compounds that target cancer cells and to prevent recognition by the reticuloendothelial system (RES), folic acid-linked poly(ethylene glycol) (FA-PEG) was further conjugated onto PAAIO-Rh123. The amounts of Rh123 and FA-PEG on the modified iron oxides were quantitatively determined by elemental analysis. The iron content was determined by inductively coupled plasma-optical emission spectrometer (ICP-OES). The particle diameters were characterized by dynamic light scattering (DLS) and transmission electron microscope (TEM). Superparamagnetism was confirmed by the superconducting quantum interference device (SQUID) magnetometer. The cellular internalization efficacy of the modified iron oxides was realized in folate-overexpressed FR(+) and folate-deficient FR(-) KB cells by flow cytometry and confocal laser scanning microscopy (CLSM). The quantitative amount of iron internalized into different harvested KB cells was measured by ICP-OES. The T(2)-weighted MR images were tested in FR(+) KB cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1878-5905
pubmed:author
pubmed:copyrightInfo
(c) 2009 Elsevier Ltd. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1707-15
pubmed:meshHeading
pubmed-meshheading:19969347-Acrylic Resins, pubmed-meshheading:19969347-Cell Death, pubmed-meshheading:19969347-Cell Line, pubmed-meshheading:19969347-Cell Survival, pubmed-meshheading:19969347-Elements, pubmed-meshheading:19969347-Ferric Compounds, pubmed-meshheading:19969347-Flow Cytometry, pubmed-meshheading:19969347-Fluorescent Dyes, pubmed-meshheading:19969347-Folic Acid, pubmed-meshheading:19969347-Humans, pubmed-meshheading:19969347-Magnetic Resonance Spectroscopy, pubmed-meshheading:19969347-Magnetics, pubmed-meshheading:19969347-Microscopy, Confocal, pubmed-meshheading:19969347-Molecular Imaging, pubmed-meshheading:19969347-Nanostructures, pubmed-meshheading:19969347-Neoplasms, pubmed-meshheading:19969347-Organ Specificity, pubmed-meshheading:19969347-Phantoms, Imaging, pubmed-meshheading:19969347-Polyethylene Glycols, pubmed-meshheading:19969347-Rhodamine 123, pubmed-meshheading:19969347-Temperature
pubmed:year
2010
pubmed:articleTitle
A specific tumor-targeting magnetofluorescent nanoprobe for dual-modality molecular imaging.
pubmed:affiliation
Faculty of Medicinal & Applied Chemistry, School of Life Science, Kaohsiung Medical University, Kaohsiung City 80708, Taiwan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't