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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2009-12-7
pubmed:abstractText
Labeled molecular markers are an important tool in molecular biology. This work presents a method for the automatic identification of molecular markers in dot blot images. The method detects the location of markers in the image and their size. An experiment was made with 6 test images, which were used to produce an additional set of 222 images with various rotation, translation, contrast and noise levels. Over 7500 markers were identified automatically and compared with reference values obtained manually. The RMS error for the marker positioning in the original test images were between 1.1 and 3.8 pixels, which is about 1/10 of the typical radius (26 pixels). The method proposed was found to be almost insensitive to grid rotation and translation, and reasonably robust to image contrast changes and presence of noise.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1557-170X
pubmed:author
pubmed:issnType
Print
pubmed:volume
2009
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6710-3
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Automatic detection of molecular markers in digital images.
pubmed:affiliation
Faculdade de Ciências, Univ. Porto, DMA, Rua do Campo Alegre 687, 4169-007 Porto, Portugal. andre.marcal@fc.up.pt
pubmed:publicationType
Journal Article