Linked Life Data

19962368

Source:http://linkedlifedata.com/resource/pubmed/id/19962368

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-1-27
pubmed:abstractText
Flavivirus pre-membrane (prM) protein is important for proper folding and secretion of envelope (E) protein. However, other functions of prM protein in relation to virus life-cycle are poorly characterized. In this study, we aimed to elucidate if dengue virus (DENV) prM protein interacts with host proteins and contributes to viral pathogenesis by screening human liver cDNA yeast two-hybrid library. Our study identified claudin-1 as a novel interacting partner of DENV prM protein. Virus production was significantly attenuated in claudin-1 knock-down cells. We showed that claudin-1 expression is up-regulated at the early phase of infection to facilitate DENV entry and down-regulated at the late stage of infection probably to prevent super-infection. Our study also demonstrated that DENV C protein played an important role in down-regulating claudin-1 expression during DENV infection.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1090-2104
pubmed:author
pubmed:copyrightInfo
Copyright 2009 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
391
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
952-7
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Novel binding between pre-membrane protein and claudin-1 is required for efficient dengue virus entry.
pubmed:affiliation
Department of Endocrinology & Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't