Linked Life Data

19958762

Source:http://linkedlifedata.com/resource/pubmed/id/19958762

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-3
pubmed:dateCreated
2010-1-27
pubmed:abstractText
The purpose of this study was to determine whether expression of inducible nitric oxide synthase (iNOS) is altered in vascular smooth muscle cells of type 2 diabetes rats. We used cultured aortic smooth muscle cells (ASMCs) isolated from male Goto-Kakizaki diabetes rats (G-K rats) aged 27-28weeks and age-matched Wistar rats (control rats). iNOS and extracellular signal-regulated kinase (ERK) were evaluated by immunoblot and/or immunochemical analyses, and NO production was evaluated by measuring NO(X) (NO(2) and NO(3)). Expression of iNOS was not detected in ASMCs of either G-K or control rats under a resting condition. Stimulation with interleukin-1beta (IL-1beta) induced iNOS expression, which was much greater in ASMCs from G-K rats than in ASMCs from control rats. When ASMCs were stimulated with IL-1beta, the number of iNOS-immunoreactive ASMCs from G-K rats increased more prominently than did the number of such ASMCs from control rats. IL-1beta-induced NO production was also much greater in ASMCs from G-K rats than in those from control rats. Both IL-1beta-induced iNOS expression and NO production in ASMCs of G-K and control rats were markedly reduced in the presence of an ERK inhibitor, U0126 or PD98059. Both basal and IL-1beta-stimulated levels of ERK activity were significantly higher in ASMCs from G-K rats than in ASMCs from control rats. The results suggest that iNOS induction is enhanced in cultured ASMCs from G-K rats and that this enhancement is associated with increased ERK activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1879-0712
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2009 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
10
pubmed:volume
629
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-6
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Enhancement of interleukin-1beta-induced iNOS expression in cultured vascular smooth muscle cells of Goto-Kakizaki diabetes rats.
pubmed:affiliation
Department of Environmental and Preventive Medicine, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan. wakabaya@hyo-med.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't