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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1977-12-29
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pubmed:abstractText |
The extreme sensitivity of chicken muscle fructose 1,6-bisphosphatase to inhibition by 5'-AMP has been utilized to develop a new method for the assay of cAMP phosphodiesterase activity. In this method, the substrate (cAMP) is first incubated with phosphodiesterase and the amount of 5'-AMP formed is then determined by measuring the degree of inhibition of fructose 1'6-bisphosphatase activity. The present method conveniently employs the spectrophotometric technique and is sensitive enough to detect the conversion of 50 pmol of cAMP to 5'-AMP in 1 ml of reaction mixture. This method is considered particularly valuable for those laboratories that are not equipped with facilities for measuring radioactivity.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-924X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
387-94
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pubmed:dateRevised |
2007-12-19
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pubmed:meshHeading |
pubmed-meshheading:199581-3',5'-Cyclic-AMP Phosphodiesterases,
pubmed-meshheading:199581-AMP Deaminase,
pubmed-meshheading:199581-Adenosine Monophosphate,
pubmed-meshheading:199581-Animals,
pubmed-meshheading:199581-Cattle,
pubmed-meshheading:199581-Chickens,
pubmed-meshheading:199581-Cyclic AMP,
pubmed-meshheading:199581-Female,
pubmed-meshheading:199581-Fructose-Bisphosphatase,
pubmed-meshheading:199581-Kinetics,
pubmed-meshheading:199581-Methods,
pubmed-meshheading:199581-Muscles,
pubmed-meshheading:199581-Spectrophotometry
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pubmed:year |
1977
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pubmed:articleTitle |
A new method for measurement of cyclic AMP phosphodiesterase activity.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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