Source:http://linkedlifedata.com/resource/pubmed/id/19958157
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2010-1-13
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pubmed:abstractText |
Interleukin (IL)-15 serves as a survival factor for a broad array of cells. Renal cells express both IL-15 and its receptor (IL-15R); however, the role of IL-15 in the kidney is yet to be determined. We examined IL-15 and IL-15R levels in sepsis-related renal injury, ischemia-reperfusion injury (IRI), and cisplatin-induced nephrotoxicity. To test the anti-apoptotic effect of IL-15, Bcl-2/Bax mRNA levels were assessed in kidneys of IL-15Ralpha(-/-) mice and in IL-15-stimulated renal epithelial cells (RECs). In addition, RECs were exposed to cisplatin and apoptosis was evaluated by TUNEL staining, caspase-3 activity, and cell cycle analysis. Intrarenal IL-15 levels decreased 24 h after initiation of all three examined pathologies by 5.8-fold (sepsis), 11-fold (IRI), and 23-fold (cisplatin-induced nephrotoxicity). Further experiments revealed that while addition of rIL-15 (1 ng/mL) to wild-type (WT) RECs increased Bcl-2/Bax ratio by 2-fold, kidneys of IL-15Ralpha(-/-) mice exhibited 4-fold lower Bcl-2/Bax ratio compared to WT mice. Accordingly, IL-15 lowered the apoptotic rate in cisplatin-treated cultured REC, and IL-15Ralpha(-/-) renal cells exhibited a higher rate of cisplatin-induced apoptosis. Furthermore, IL-15 levels negatively correlated with BUN of cisplatin-treated mice (R = -0.69, P = 0.003), suggesting that a decline in renal-derived IL-15 is detrimental to renal cell survival and kidney function during pathological stress.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Cisplatin,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-15,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-15
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1557-7465
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
30
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-8
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pubmed:meshHeading |
pubmed-meshheading:19958157-Animals,
pubmed-meshheading:19958157-Apoptosis,
pubmed-meshheading:19958157-Caspase 3,
pubmed-meshheading:19958157-Cells, Cultured,
pubmed-meshheading:19958157-Cisplatin,
pubmed-meshheading:19958157-Epithelial Cells,
pubmed-meshheading:19958157-Interleukin-15,
pubmed-meshheading:19958157-Kidney Diseases,
pubmed-meshheading:19958157-Mice,
pubmed-meshheading:19958157-Mice, Inbred C57BL,
pubmed-meshheading:19958157-Mice, Knockout,
pubmed-meshheading:19958157-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:19958157-Receptors, Interleukin-15,
pubmed-meshheading:19958157-Reperfusion Injury,
pubmed-meshheading:19958157-Sepsis
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pubmed:year |
2010
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pubmed:articleTitle |
Association between renal injury and reduced interleukin-15 and interleukin-15 receptor levels in acute kidney injury.
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pubmed:affiliation |
Clinical Biochemistry Department, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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