Linked Life Data

19957128

Source:http://linkedlifedata.com/resource/pubmed/id/19957128

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2009-12-3
pubmed:abstractText
To follow the cell division cycle in the living state, certain biological activity or morphological changes must be monitored keeping the cells intact. Mitotic events from prophase to telophase are well defined by morphology or movement of chromatin, nuclear envelope, centrosomes, and/or spindles. To paint or simultaneously visualize these mitotic subcellular structures, we have been using ECFP-histone H3 for chromatin and chromosomes, EGFP-Aurora-A for centrosomes and kinetochore spindles and DsRed-fused truncated peptide of importin alpha for the outer surface of nuclear envelope as living cell markers. Time-lapse images from prophase through to early G1 phase can be obtained by constructing a triple-fluorescent cell line (Sugimoto et al., Cell Struct. Funct. 27, 457-467, 2002). Here, we describe the multicolor imaging of mitosis of a human breast cancer cell line, MDA435, and a further application to characterizing the apoptotic chromatin condensation process in isolated nuclei by simultaneously visualizing kinetochores with EGFP and chromatin with a fluorescent dye, SYTO 59.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1940-6029
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
591
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
135-46
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Imaging of mitotic cell division and apoptotic intra-nuclear processes in multicolor.
pubmed:affiliation
Live Cell Imaging Institute, Osaka Prefecture University, Sakai, Osaka, Japan.
pubmed:publicationType
Journal Article