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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1991-3-27
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pubmed:databankReference |
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pubmed:abstractText |
We previously identified a protein related to the expression of the ilvGMEDA cluster of Escherichia coli K-12. It was observed that this ilv-related protein was produced at higher levels in UV irradiated cells infected with lambda dilvGMEDA phage with specific ilvG mutations (ValR), compared to phage carrying the wild-type(ValS) ilvG allele. The gene encoding this protein was further localized to a region between rrnC and ilvGMEDA by analyzing restriction fragment subsets in maxicells. We have now determined the nucleotide (nt) sequence of the 3.5-kb segment between rrnC and ilvGMEDA, and two open reading frames (ORFs) are present in the region expected to contain the ilv-related gene. These ORFs predicts Mrs of 18,751 (ORFI) and 20,085 (ORFII) Da, and both ORFs have a strong probability to encode proteins based on codon frequency analysis. Maxicell analysis revealed that a 1319-bp HindIII-SmaI fragment containing ORFI encodes the ilv-related peptide. We deleted a ClaI fragment that removed a portion of ORFI encoding the C-terminal region of the peptide, and maxicell analysis revealed a decrease in the size of the protein produced in accord with the prediction. RNA slot blots and Northern blots were used to characterize transcripts encoding ORFI. A transcript initiated 112 nt from the ilvGp2 promoter, but proceeding in the opposite direction, may encode the ORFI peptide.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0378-1119
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
97
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
21-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1995430-Amino Acid Sequence,
pubmed-meshheading:1995430-Bacterial Proteins,
pubmed-meshheading:1995430-Base Sequence,
pubmed-meshheading:1995430-Blotting, Northern,
pubmed-meshheading:1995430-Chromosome Mapping,
pubmed-meshheading:1995430-Chromosomes, Bacterial,
pubmed-meshheading:1995430-Codon,
pubmed-meshheading:1995430-Escherichia coli,
pubmed-meshheading:1995430-Escherichia coli Proteins,
pubmed-meshheading:1995430-Isoleucine,
pubmed-meshheading:1995430-Molecular Sequence Data,
pubmed-meshheading:1995430-Open Reading Frames,
pubmed-meshheading:1995430-Restriction Mapping,
pubmed-meshheading:1995430-Transcription, Genetic,
pubmed-meshheading:1995430-Valine
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pubmed:year |
1991
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pubmed:articleTitle |
Sequence and transcriptional activity of the Escherichia coli K-12 chromosome region between rrnC and ilvGMEDA.
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pubmed:affiliation |
Department of Chemistry, City College of New York, NY 10031.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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