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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2010-1-26
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pubmed:abstractText |
Gold nanocages with localized surface plasmon resonance peaks in the near-infrared region exhibited a broad two-photon photoluminescence band extending from 450 to 650 nm when excited by a Ti:sapphire laser at 800 nm. The bright luminescence makes it possible to explore the use of Au nanocages as a new class of optical imaging agents for two-photon microscopy. In this work, we have demonstrated the use of two-photon microscopy as a convenient tool to directly examine the uptake of antibody-conjugated and PEGylated Au nanocages by U87MGwtEGFR cells. We have also correlated the results from two-photon microscopy with the data obtained by inductively coupled plasma mass spectrometry. Combined together, these results indicate that the antibody-conjugated Au nanocages were attached to the surface of the cells through antibody-antigen binding and then internalized into the cells via receptor-mediated endocytosis. The cellular uptake process was dependent on a number of parameters, including incubation time, incubation temperature, size of the Au nanocages, and the number of antibodies immobilized on each nanocage.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19954236-14597719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19954236-15038743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19954236-15469240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19954236-15755097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19954236-15972807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19954236-16239346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19954236-16464114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19954236-16608261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19954236-16852648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19954236-17335272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19954236-17430005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19954236-18020475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19954236-18384724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19954236-18570442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19954236-18703609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19954236-19072058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19954236-19075560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19954236-19199477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19954236-19271715
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1936-086X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
26
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
35-42
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
pubmed-meshheading:19954236-Animals,
pubmed-meshheading:19954236-Antibodies,
pubmed-meshheading:19954236-Biological Transport,
pubmed-meshheading:19954236-Cell Line, Tumor,
pubmed-meshheading:19954236-Gold,
pubmed-meshheading:19954236-Humans,
pubmed-meshheading:19954236-Mass Spectrometry,
pubmed-meshheading:19954236-Metal Nanoparticles,
pubmed-meshheading:19954236-Microscopy,
pubmed-meshheading:19954236-Photons,
pubmed-meshheading:19954236-Receptor, Epidermal Growth Factor,
pubmed-meshheading:19954236-Spectrophotometry, Ultraviolet
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pubmed:year |
2010
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pubmed:articleTitle |
Quantifying the cellular uptake of antibody-conjugated Au nanocages by two-photon microscopy and inductively coupled plasma mass spectrometry.
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pubmed:affiliation |
Department of Biomedical Engineering, Washington University, St Louis, Missouri 63130, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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