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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1991-3-27
|
| pubmed:abstractText |
The use of an electric freezer for cryostorage of human semen is described. A simple method for semen freezing in liquid nitrogen after dilution with 7.5% glycerol was used. Thawing for quality analysis revealed only a small decrease (10%) in post-thaw sperm motility (59 +/- 1.4 vs. 53 +/- 1.8%; mean +/- SE, n = 12, P less than 10(-5)) and 14% in post-thaw sperm vitality (85.0 +/- 1.3 vs. 72.9 +/- 1.8%). After the freezing process, the samples, three of each donor, were cryopreserved in a regular electric freezer which maintained temperatures in the range of -85 degrees C (+/- 2 degrees C). The samples were stored for 1 week, 2 and 6 months, thawed and then assayed for motility and vitality. No effect of storage was found for a period up to 2 months. An additional decrease of 17.2% in sperm motility and 18% in sperm vitality were noted only after 6 months of preservation. The final motility and vitality rates of these sperm samples were 44 +/- 2.4 and 60 +/- 3.0%, respectively. According to these results, in cases of sperm storage for limited periods, it is recommended to cryopreserve human semen by the use of a combination of freezing in liquid nitrogen and storage of the samples in an electric freezer at -85 degrees C.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0301-2115
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
30
|
| pubmed:volume |
38
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
141-4
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:1995382-Analysis of Variance,
pubmed-meshheading:1995382-Cryopreservation,
pubmed-meshheading:1995382-Freezing,
pubmed-meshheading:1995382-Humans,
pubmed-meshheading:1995382-Male,
pubmed-meshheading:1995382-Semen Preservation,
pubmed-meshheading:1995382-Sperm Banks,
pubmed-meshheading:1995382-Sperm Motility
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
The use of an electric freezer in human semen banking.
|
| pubmed:affiliation |
Serlin Maternity Hospital, Tel Aviv Medical Center, Sackler Medical School, Tel Aviv University, Israel.
|
| pubmed:publicationType |
Journal Article
|