Linked Life Data

19950181

Source:http://linkedlifedata.com/resource/pubmed/id/19950181

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-2-3
pubmed:abstractText
Lymphoid tissue inducer cells (LTi) play an important role in the development of lymphoid tissue in embryos. Adult CD4(+)CD3(-) LTi-like cells present a similar phenotype and gene expression to their embryonic counterpart and have important roles in CD4(+) T-cell memory and lymphoid tissue recovery following viral infection. However, adult LTi-like cells are heterogeneous populations and the factors that regulate their survival and accumulation within secondary lymphoid organs remain unclear, in particular whether the T-zone stroma is involved. Here we report the identification and characterization of a distinct subset of podoplanin(+) murine splenic stromal cells that support adult LTi-like cell survival. We have identified and isolated CD45(-)podoplanin(+) stromal cell populations which have a similar but distinct phenotype to T-zone reticular cells in LN. CD45(-)podoplanin(+) fibroblast-like cells mediate LTi-like cell survival in vitro; surprisingly this was not dependent upon IL-7 as revealed through blocking Ab experiments and studies using LTi-like cells unable to respond to gamma chain cytokines. Our findings show that adult LTi-like cells require extrinsic signals from podoplanin(+) splenic stromal cells to survive and suggest that IL-7 is not necessary to mediate their survival in the adult spleen.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1521-4141
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
359-65
pubmed:meshHeading
pubmed-meshheading:19950181-Animals, pubmed-meshheading:19950181-Antigens, CD45, pubmed-meshheading:19950181-Cell Survival, pubmed-meshheading:19950181-Cells, Cultured, pubmed-meshheading:19950181-Female, pubmed-meshheading:19950181-Flow Cytometry, pubmed-meshheading:19950181-Fluorescent Antibody Technique, pubmed-meshheading:19950181-Interleukin-7, pubmed-meshheading:19950181-Lymphoid Tissue, pubmed-meshheading:19950181-Male, pubmed-meshheading:19950181-Membrane Glycoproteins, pubmed-meshheading:19950181-Mice, pubmed-meshheading:19950181-Mice, Inbred C57BL, pubmed-meshheading:19950181-Mice, Knockout, pubmed-meshheading:19950181-Mice, Transgenic, pubmed-meshheading:19950181-Receptors, Interleukin-7, pubmed-meshheading:19950181-Spleen, pubmed-meshheading:19950181-Stromal Cells, pubmed-meshheading:19950181-T-Lymphocytes, Helper-Inducer, pubmed-meshheading:19950181-Time Factors
pubmed:year
2010
pubmed:articleTitle
Splenic stromal cells mediate IL-7 independent adult lymphoid tissue inducer cell survival.
pubmed:affiliation
School of Immunity and Infection, MRC Centre for Immune Regulation, Institute for Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't