Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-2-1
pubmed:abstractText
Previously we described that bone morphogenetic protein-7 (BMP7) could protect prostate cancer C4-2B cells from serum starvation-induced apoptosis via survivin induction. Here, for the first time, we identify Runx2 as a key regulator of survivin transcription. In C4-2B cells grown normally, suppression of Runx2 reduced survivin expression. Using ChIP assays, two regions of the survivin promoter, -1953 to -1812 (I) and -1485 to -1119 (II) encompassing consensus Runx-binding sites were examined. Runx2 was found to be associated with both regions, with a stronger affinity to region-I. In serum-starved cells neither region was occupied, but BMP7 restored association to region-II and not region-I. In reporter assays, transcription activity by BMP7 was significantly reduced when sequences including binding sites of region-II were deleted. Additionally, Runx2 expression was enhanced by BMP7 in these cells. Along with a strong survivin expression, a trend in increased Runx2 expression in human prostate cancer cells and tissues was noted. In the conditional Pten-knockout mouse, Runx2 level increased with growth of prostate tumor. The data define a novel role of Runx2 in regulating survivin expression in malignant epithelial cells and identify it as a critical factor in BMP signaling that protects cancer cells against apoptosis.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-10521447, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-10531362, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-10567210, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-10844590, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-11073979, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-11263661, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-11932250, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-12060751, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-12231506, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-12393476, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-12539225, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-12746842, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-12890574, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-14522255, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-14991870, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-15163300, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-15231660, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-15537544, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-15829516, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-15861517, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-15864279, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-15994952, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-16166639, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-16237704, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-16613856, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-16618753, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-16892037, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-17671224, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-17876328, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-18075512, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-18172312, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-18204048, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-18263612, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-18500170, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-18728344, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-19264160, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-2263636, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-7891692, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-8413232, http://linkedlifedata.com/resource/pubmed/commentcorrection/19949374-9399875
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1530-0307
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
90
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
222-33
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Runx2 regulates survivin expression in prostate cancer cells.
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