Linked Life Data

19948658

Source:http://linkedlifedata.com/resource/pubmed/id/19948658

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 2
pubmed:dateCreated
2010-1-18
pubmed:abstractText
Atrial natriuretic peptide (ANP) via its guanylyl cyclase-A (GC-A) receptor participates in regulation of arterial blood pressure and vascular volume. Previous studies demonstrated that concerted renal diuretic/natriuretic and endothelial permeability effects of ANP cooperate in intravascular volume regulation. We show that the microvascular endothelial contribution to the hypovolaemic action of ANP can be measured by the magnitude of the ANP-induced increase in blood-to-tissue albumin transport, measured as plasma albumin clearance corrected for intravascular volume change, relative to the corresponding increase in ANP-induced renal water excretion. We used a two-tracer method with isotopically labelled albumin to measure clearances in skin and skeletal muscle of: (i) C57BL6 mice; (ii) mice with endothelium-restricted deletion of GC-A (floxed GC-A x tie2-Cre: endothelial cell (EC) GC-A knockout (KO)); and (iii) control littermates (floxed GC-A mice with normal GC-A expression levels). Comparison of albumin clearances in hypervolaemic EC GC-A KO mice with normovolaemic littermates demonstrated that skeletal muscle albumin clearance with ANP treatment accounts for at most 30% of whole body clearance required for ANP to regulate plasma volume. Skin microcirculation responded to ANP similarly. Measurements of permeability to a high molecular mass contrast agent (35 kD Gadomer) by dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) enabled repeated measures in individual animals and confirmed small increases in muscle and skin microvascular permeability after ANP. These quantitative methods will enable further evaluation of the contribution of ANP-dependent microvascular beds (such as gastro-intestinal tract) to plasma volume regulation.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948658-10390517, http://linkedlifedata.com/resource/pubmed/commentcorrection/19948658-11390268, http://linkedlifedata.com/resource/pubmed/commentcorrection/19948658-12865659, http://linkedlifedata.com/resource/pubmed/commentcorrection/19948658-15718260, http://linkedlifedata.com/resource/pubmed/commentcorrection/19948658-15931381, http://linkedlifedata.com/resource/pubmed/commentcorrection/19948658-15931395, http://linkedlifedata.com/resource/pubmed/commentcorrection/19948658-16126813, http://linkedlifedata.com/resource/pubmed/commentcorrection/19948658-16253523, http://linkedlifedata.com/resource/pubmed/commentcorrection/19948658-18293091, http://linkedlifedata.com/resource/pubmed/commentcorrection/19948658-18450968, http://linkedlifedata.com/resource/pubmed/commentcorrection/19948658-19142210, http://linkedlifedata.com/resource/pubmed/commentcorrection/19948658-19218620, http://linkedlifedata.com/resource/pubmed/commentcorrection/19948658-20080514, http://linkedlifedata.com/resource/pubmed/commentcorrection/19948658-2943959, http://linkedlifedata.com/resource/pubmed/commentcorrection/19948658-8650246, http://linkedlifedata.com/resource/pubmed/commentcorrection/19948658-9287305, http://linkedlifedata.com/resource/pubmed/commentcorrection/19948658-9789255
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1469-7793
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
588
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
325-39
pubmed:dateRevised
2011-7-20
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Atrial natriuretic peptide modulation of albumin clearance and contrast agent permeability in mouse skeletal muscle and skin: role in regulation of plasma volume.
pubmed:affiliation
Department of Physiology and Membrane Biology, School of Medicine, University of California, Davis, Davis, CA 95616, USA. fecurry@ucdavis.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural