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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2009-12-1
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pubmed:abstractText |
Nodal and Notch signaling pathways play essential roles in vertebrate development. Through a yeast two-hybrid screening, we identified Notch3 as a candidate binding partner of the Nodal coreceptor Cripto-1. Coimmunoprecipitation analysis confirmed the binding of Cripto-1 with all four mammalian Notch receptors. Deletion analyses revealed that the binding of Cripto-1 and Notch1 is mediated by the Cripto-1/FRL-1/Cryptic domain of Cripto-1 and the C-terminal region of epidermal growth factor-like repeats of Notch1. Binding of Cripto-1 to Notch1 occurred mainly in the endoplasmic reticulum-Golgi network. Cripto-1 expression resulted in the recruitment of Notch1 protein into lipid raft microdomains and enhancement of the furin-like protein convertase-mediated proteolytic maturation of Notch1 (S1 cleavage). Enhanced S1 cleavage resulted in the sensitization to ligand-induced activation of Notch signaling. In addition, knockdown of Cripto-1 expression in human and mouse embryonal carcinoma cells desensitized the ligand-induced Notch signaling activation. These results suggest a novel role of Cripto-1 in facilitating the posttranslational maturation of Notch receptors.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948478-11150244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948478-11595185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948478-11784114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948478-12760378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948478-14499649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948478-15561108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948478-15949532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948478-16123806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948478-17287255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948478-17296795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948478-17409286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948478-17720976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948478-17925387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948478-18268105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948478-18347015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948478-18445292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948478-18772886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948478-18930707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948478-19155199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948478-2792079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19948478-9653148
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Efemp2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Matrix Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GPI-Linked Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Notch3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Notch1,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Notch,
http://linkedlifedata.com/resource/pubmed/chemical/TDGF1 protein, human
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1540-8140
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pubmed:author |
pubmed-author:BiancoCaterinaC,
pubmed-author:CallahanRobertR,
pubmed-author:CastroNadia PNP,
pubmed-author:GonzalesMonicaM,
pubmed-author:LeeJoseph MJM,
pubmed-author:NagaokaTadahiroT,
pubmed-author:RangelMaria CristinaMC,
pubmed-author:SakamotoKeiK,
pubmed-author:SalomonDavid SDS,
pubmed-author:SunYoupingY,
pubmed-author:WatanabeKazuhideK
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pubmed:issnType |
Electronic
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pubmed:day |
2
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pubmed:volume |
187
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
343-53
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:19948478-Amino Acid Motifs,
pubmed-meshheading:19948478-Animals,
pubmed-meshheading:19948478-Binding Sites,
pubmed-meshheading:19948478-CHO Cells,
pubmed-meshheading:19948478-COS Cells,
pubmed-meshheading:19948478-Cells, Cultured,
pubmed-meshheading:19948478-Cercopithecus aethiops,
pubmed-meshheading:19948478-Cricetinae,
pubmed-meshheading:19948478-Cricetulus,
pubmed-meshheading:19948478-Epidermal Growth Factor,
pubmed-meshheading:19948478-Extracellular Matrix Proteins,
pubmed-meshheading:19948478-GPI-Linked Proteins,
pubmed-meshheading:19948478-Gene Library,
pubmed-meshheading:19948478-Humans,
pubmed-meshheading:19948478-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:19948478-Membrane Glycoproteins,
pubmed-meshheading:19948478-Membrane Microdomains,
pubmed-meshheading:19948478-Mice,
pubmed-meshheading:19948478-Neoplasm Proteins,
pubmed-meshheading:19948478-Protein Interaction Mapping,
pubmed-meshheading:19948478-Receptor, Notch1,
pubmed-meshheading:19948478-Receptors, Notch,
pubmed-meshheading:19948478-Signal Transduction,
pubmed-meshheading:19948478-Two-Hybrid System Techniques
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pubmed:year |
2009
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pubmed:articleTitle |
Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1.
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pubmed:affiliation |
Mammary Biology and Tumorigenesis Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Intramural
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