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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2010-1-27
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pubmed:abstractText |
We previously demonstrated that ceramide-1-phosphate (C1P) stimulates fibroblast and macrophage proliferation, but the mechanisms involved in this action have only been partially described. Here we demonstrate that C1P induces translocation of protein kinase C-alpha (PKC-alpha) from the soluble to the membrane fraction of bone marrow-derived macrophages. Translocation of this enzyme was accompanied by its phosphorylation on Ser 657 residue. Activation of PKC-alpha was independent of prior stimulation of phosphatidylinositol-dependent or phosphatidylcholine-dependent phospholipase C activities, but required activation of sphingomyelin synthesis. Inhibition of PKC-alpha activation also blocked C1P-stimulated macrophage proliferation indicating that this enzyme is essential for the mitogenic effect of C1P.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1873-3468
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pubmed:author |
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pubmed:copyrightInfo |
2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
5
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pubmed:volume |
584
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
517-24
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pubmed:meshHeading |
pubmed-meshheading:19948174-Animals,
pubmed-meshheading:19948174-Blotting, Western,
pubmed-meshheading:19948174-Cell Proliferation,
pubmed-meshheading:19948174-Cells, Cultured,
pubmed-meshheading:19948174-Ceramides,
pubmed-meshheading:19948174-Enzyme Activation,
pubmed-meshheading:19948174-Female,
pubmed-meshheading:19948174-Inositol Phosphates,
pubmed-meshheading:19948174-Mice,
pubmed-meshheading:19948174-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:19948174-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:19948174-Phosphorylation,
pubmed-meshheading:19948174-Protein Kinase C-alpha,
pubmed-meshheading:19948174-Protein Transport,
pubmed-meshheading:19948174-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:19948174-Sphingomyelins,
pubmed-meshheading:19948174-Transferases (Other Substituted Phosphate Groups)
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pubmed:year |
2010
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pubmed:articleTitle |
Activation of protein kinase C-alpha is essential for stimulation of cell proliferation by ceramide 1-phosphate.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of the Basque Country, Bilbao, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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