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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 10
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pubmed:dateCreated |
2010-10-8
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pubmed:databankReference |
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pubmed:abstractText |
A halophilic archaeon, strain RO1-6(T), was isolated from a marine solar saltern in eastern China. Cells of strain RO1-6(T) were pleomorphic and motile and stained Gram-negative. Strain RO1-6(T) grew well on complex medium and colonies were red-pigmented. It was able to grow at 20-50°C (optimum 37°C), in 2.1-5.1M NaCl (optimum 3.9 M NaCl), in 0.05-0.70 M MgCl? (optimum 0.30 M MgCl?) and at pH 6.5-8.0 (optimum pH 7.0). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 12?% (w/v). The major polar lipids of strain RO1-6(T) were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two glycolipids that were chromatographically identical to S-DGD-1 and S?-DGD. The 16S rRNA gene sequence of strain RO1-6(T) showed similarities of 96.9 and 96.4?% to those of the type strains of Halosarcina pallida and Halogeometricum borinquense, respectively, members of the most closely related recognized genera within the family Halobacteriaceae. The DNA G+C content of strain RO1-6(T) was 61.2 mol%. Phenotypic characterization and phylogenetic analysis revealed that strain RO1-6(T) is related to Halosarcina pallida and represents a novel species of the genus Halosarcina, for which the name Halosarcina limi sp. nov. is proposed; the type strain is RO1-6(T) (=CGMCC 1.8711(T) =JCM 16054(T)).
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Archaeal,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Ribosomal,
http://linkedlifedata.com/resource/pubmed/chemical/Glycolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Magnesium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/Pigments, Biological,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Archaeal,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 16S,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Chloride
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1466-5034
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
60
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2462-3466
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pubmed:meshHeading |
pubmed-meshheading:19946053-Base Composition,
pubmed-meshheading:19946053-China,
pubmed-meshheading:19946053-Cluster Analysis,
pubmed-meshheading:19946053-DNA, Archaeal,
pubmed-meshheading:19946053-DNA, Ribosomal,
pubmed-meshheading:19946053-Genes, rRNA,
pubmed-meshheading:19946053-Geologic Sediments,
pubmed-meshheading:19946053-Glycolipids,
pubmed-meshheading:19946053-Halobacteriaceae,
pubmed-meshheading:19946053-Hydrogen-Ion Concentration,
pubmed-meshheading:19946053-Locomotion,
pubmed-meshheading:19946053-Magnesium Chloride,
pubmed-meshheading:19946053-Molecular Sequence Data,
pubmed-meshheading:19946053-Phospholipids,
pubmed-meshheading:19946053-Phylogeny,
pubmed-meshheading:19946053-Pigments, Biological,
pubmed-meshheading:19946053-RNA, Archaeal,
pubmed-meshheading:19946053-RNA, Ribosomal, 16S,
pubmed-meshheading:19946053-Sequence Analysis, DNA,
pubmed-meshheading:19946053-Sequence Homology, Nucleic Acid,
pubmed-meshheading:19946053-Sodium Chloride,
pubmed-meshheading:19946053-Temperature
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pubmed:year |
2010
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pubmed:articleTitle |
Halosarcina limi sp. nov., a halophilic archaeon from a marine solar saltern, and emended description of the genus Halosarcina.
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pubmed:affiliation |
School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China. cuihenglin@sohu.com
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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