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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2010-1-27
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pubmed:abstractText |
We report the preparation of size-defined [(15)N]N-acetylheparosan oligosaccharides from Escherichia coli-derived (15)N-enriched N-acetylheparosan. Optimized growth conditions of E. coli in minimal media containing (15)NH(4)Cl yielded [(15)N]N-acetylheparosan on a preparative scale. Depolymerization of [(15)N]N-acetylheparosan by heparitinase I yielded resolvable, even-numbered oligosaccharides ranging from disaccharide to icosaccharide. Anion-exchange chromatography-assisted fractionation afforded size-defined [(15)N]N-acetylheparosan oligosaccharides identifiable by ESI-TOFMS. These isotopically labeled oligosaccharides will prove to be valuable research tools for the chemoenzymatic synthesis of heparin and heparan sulfate oligosaccharides and for the study of their structural biology.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-10872465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-11473122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-12121115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-12244479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-12491369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-14528313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-14531682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-15658847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-16330006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-17460664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-17598000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-17884631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-18767845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-1902083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-19402671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-4586320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-4817700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-7018909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-7805054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-7961837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-8836040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-9151776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19945695-9377090
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1873-426X
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pubmed:author |
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pubmed:copyrightInfo |
2009 Elsevier Ltd. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
26
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pubmed:volume |
345
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
250-6
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pubmed:dateRevised |
2011-7-20
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pubmed:meshHeading |
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pubmed:year |
2010
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pubmed:articleTitle |
Preparation and characterization of (15)N-enriched, size-defined heparan sulfate precursor oligosaccharides.
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pubmed:affiliation |
Interdepartmental Program in Neuroscience, University of Utah, Salt Lake City, 20 North 1900 East, MREB #401, UT 84132, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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