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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0027022,
umls-concept:C0040649,
umls-concept:C0077274,
umls-concept:C0086418,
umls-concept:C0162638,
umls-concept:C0205263,
umls-concept:C0596311,
umls-concept:C1330957,
umls-concept:C1334291,
umls-concept:C1366587,
umls-concept:C1704689
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pubmed:issue |
2
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pubmed:dateCreated |
2010-4-5
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pubmed:abstractText |
The discovery of oncogene addiction in myeloproliferative disorders (MPDs) driven by the gain-of-function mutant Jak2V617F has attracted intense interest in targeted therapy for MPDs. In this report, we demonstrate that triptolide potently downregulated the transcription of Jak2 by inhibiting the activity of RNA polymerase. Triptolide inhibited the in vitro and in vivo growth of tumor cells harboring Jak2V617F. Triptolide induced abundant apoptosis with a prominent decline of Bcl-2, Bcl-X(L), survivin and Mcl-1. As well, triptolide induced caspase-3-dependent Mcl-1 cleavage, which may potentiate apoptosis. These findings suggest that triptolide is a promising agent to kill Jak2V617F-harboring cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Inflammatory Agents...,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Diterpenes,
http://linkedlifedata.com/resource/pubmed/chemical/Epoxy Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/JAK2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Janus Kinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Phenanthrenes,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/myeloid cell leukemia sequence 1...,
http://linkedlifedata.com/resource/pubmed/chemical/triptolide
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1872-7980
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
28
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pubmed:volume |
291
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
246-55
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pubmed:meshHeading |
pubmed-meshheading:19942343-Amino Acid Substitution,
pubmed-meshheading:19942343-Anti-Inflammatory Agents, Non-Steroidal,
pubmed-meshheading:19942343-Apoptosis,
pubmed-meshheading:19942343-Caspase 3,
pubmed-meshheading:19942343-Cell Cycle,
pubmed-meshheading:19942343-Cell Line, Tumor,
pubmed-meshheading:19942343-Cycloheximide,
pubmed-meshheading:19942343-DNA Primers,
pubmed-meshheading:19942343-Diterpenes,
pubmed-meshheading:19942343-Epoxy Compounds,
pubmed-meshheading:19942343-Flow Cytometry,
pubmed-meshheading:19942343-Humans,
pubmed-meshheading:19942343-Janus Kinase 2,
pubmed-meshheading:19942343-Leukemia, Erythroblastic, Acute,
pubmed-meshheading:19942343-Myeloproliferative Disorders,
pubmed-meshheading:19942343-Phenanthrenes,
pubmed-meshheading:19942343-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:19942343-RNA, Messenger,
pubmed-meshheading:19942343-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19942343-Signal Transduction,
pubmed-meshheading:19942343-Transcription, Genetic
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pubmed:year |
2010
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pubmed:articleTitle |
Triptolide inhibits Jak2 transcription and induces apoptosis in human myeloproliferative disorder cells bearing Jak2V617F through caspase-3-mediated cleavage of Mcl-1.
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pubmed:affiliation |
Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, PR China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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