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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-11-27
pubmed:abstractText
The complete genomic sequencing and characterization of Bovine viral diarrhea virus (BVDV) isolate XJ-04 originated from cattle in China was described. The genomic RNA of the isolate was 12,284 nt long and contained short 5'- untranslated region (UTR), 3'-non-coding regions (NCR), and one open reading frame (ORF) encoding a large polyprotein of 3,895 amino acids with 20 potential N-glycosylation sites. The identity of the isolate XJ-04 with reference strains NADL (BVDV-1) and 890 (BVDV-2) in autoprotease (N(pro)) gene and structural genes (C, E(rns), E1, E2) was analyzed. The percentage of nt and aa identity in analyzed genes indicated that the isolate XJ-04 was closer to the reference strain 890 (BVDV-2) than to NADL (BVDV-1). Phylogenetic analysis revealed that the isolate belonged to BVDV-2a subtype. Furthermore, comparison analysis indicated that the isolate XJ-04 did not contain any genomic insertions that can be directly related to the cytopathic phenotype.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0001-723X
pubmed:author
pubmed:issnType
Print
pubmed:volume
53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
197-202
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Genomic sequencing and characterization of a Chinese isolate of Bovine viral diarrhea virus 2.
pubmed:affiliation
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, P.R. China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't